Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach
文献类型：期刊论文
| 作者 | Tian, Ruijun; Jiang, Xinning; Li, Xin; Jiang, Xiaogang; Feng, Shun; Xu, Songyun; Han, Guanghui; Ye, Mingliang; Zou, Hanfa |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2006-11-17 |
| 卷号 | 1134期号:1-2页码:134-142 |
| 关键词 | chemiproteomics library screening interacting proteins p-aminobenzamidine trypsin-like serine proteases human plasma |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | in this study, a gel free chemiproteomic method based on chromatography was developed and applied for the biological fingerprinting analysis of complex biological system. p-aminobenzamidine (aba), an inhibitor of trypsin-like serine proteases, was immobilized for characterizing their interacting proteins in human plasma. by the proteomic analysis method, 214 proteins were identified with obvious affinity to the immobilized aba. by searching the sequences of above proteins with consensus patterns of the two active sites, seven proteins belong to trypsin-like serine protease group were found. based on the gene ontology annotation, the identified trypsin-like serine proteases have the function of catalytic activity and calcium ion binding, and are mainly involved in the biological process of blood coagulation. eight more other proteins related to calcium ion binding and blood coagulation were found. nearly all of these proteins cannot be identified by directly analyzing the plasma sample demonstrating the chemiproteomics a useful approach to characterize interacting proteins in the low abundance range. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance affinity-chromatography ; protein interactions ; chemical proteomics ; purification ; binding ; identification ; strategies ; system ; target |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242059400017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97437]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Ruijun,Jiang, Xinning,Li, Xin,et al. Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach[J]. journal of chromatography a,2006,1134(1-2):134-142. |
| APA | Tian, Ruijun.,Jiang, Xinning.,Li, Xin.,Jiang, Xiaogang.,Feng, Shun.,...&Zou, Hanfa.(2006).Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach.journal of chromatography a,1134(1-2),134-142. |
| MLA | Tian, Ruijun,et al."Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach".journal of chromatography a 1134.1-2(2006):134-142. |
入库方式： OAI收割
来源：大连化学物理研究所
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