Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes
文献类型：期刊论文
| 作者 | Hu, Ligang; Jiang, Guibin; Xu, Songyun; Pan, Chensong; Zou, Hanfa |
| 刊名 | journal of the american society for mass spectrometry
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 17期号:11页码:1616-1619 |
| ISSN号 | 1044-0305 |
| 产权排序 | 2;3 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. the described method employs a new matrix of oxidized carbon nanotubes. compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. we quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, analytical ; chemistry, physical ; spectroscopy |
| 研究领域[WOS] | chemistry ; spectroscopy |
| 关键词[WOS] | flight mass-spectrometry ; desorption/ionization ; system |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242242900017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97443]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ligang,Jiang, Guibin,Xu, Songyun,et al. Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes[J]. journal of the american society for mass spectrometry,2006,17(11):1616-1619. |
| APA | Hu, Ligang,Jiang, Guibin,Xu, Songyun,Pan, Chensong,&Zou, Hanfa.(2006).Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes.journal of the american society for mass spectrometry,17(11),1616-1619. |
| MLA | Hu, Ligang,et al."Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes".journal of the american society for mass spectrometry 17.11(2006):1616-1619. |
入库方式： OAI收割
来源：大连化学物理研究所
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