Synthesis of silica-based benzeneboronic acid affinity materials and application as pre-column in coupled-column high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Li, Fanglou; Zhao, Xinjie; Wang, Wenzhao; Xu, Guowang |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2006-11-24 |
| 卷号 | 580期号:2页码:181-187 |
| 关键词 | benzeneboronic acid-substituted silica coupled-column high-performance affinity chromatography high-performance liquid chromatography direct analysis urinary nucleosides |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | three silica-based benzeneboronic acid affinity materials were synthesized by using an in-aminobenzeneboronic acid as the ligand and using three different spacer arms. under high-pressure, three affinity pre-columns were packed with these materials and the retention of every affinity pre-column with 11 urinary nucleosides was studied. with different spacer arms of boronic acid-substituted silica materials, the absorption to vicinal alcohols (cis-diols) and stability under acidic elution conditions are of great difference. coupled-column liquid chromatographic methods for the direct analysis of urinary nucleosides were respectively established. as a result, two of three affinity pre-columns showed good chromatographic property in the on-line analysis of urinary nucleosides. the coupled-column system including pre-column i is the best with excellent linearity (r-2 > 0.995), good recoveries (85.6-96.9%) and reproducibility (r.s.d.: 1.01-4.02%). the pre-column 1 could at least endure 150 repetitive injections of a 100 mu l urinary sample. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | biological-fluids ; nucleosides |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242304800009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97599]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fanglou,Zhao, Xinjie,Wang, Wenzhao,et al. Synthesis of silica-based benzeneboronic acid affinity materials and application as pre-column in coupled-column high-performance liquid chromatography[J]. analytica chimica acta,2006,580(2):181-187. |
| APA | Li, Fanglou,Zhao, Xinjie,Wang, Wenzhao,&Xu, Guowang.(2006).Synthesis of silica-based benzeneboronic acid affinity materials and application as pre-column in coupled-column high-performance liquid chromatography.analytica chimica acta,580(2),181-187. |
| MLA | Li, Fanglou,et al."Synthesis of silica-based benzeneboronic acid affinity materials and application as pre-column in coupled-column high-performance liquid chromatography".analytica chimica acta 580.2(2006):181-187. |
入库方式： OAI收割
来源：大连化学物理研究所
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