Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit
文献类型：期刊论文
| 作者 | Shi, XZ; Li, JH; Li, AY; Lv, S; Xu, GW |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2006-04-13 |
| 卷号 | 834期号:1-2页码:122-127 |
| 关键词 | microsatellite instability loss of heterozygosity capillary electrophoresis |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | microsatellite instability (msi) and loss of heterozygosity (loh), the alteration in length and strength of short tandem repeat sequences are an important molecular characteristic of many human tumors. msi and loh analysis has become an attractive method for diagnostic and tumor research purposes. a method for the simultaneous analysis of msi and loh at the five microsatellite loci (bat-26, d17s261, d3s1283, d2s123 and d3s133s d3s1611) was developed employing a cheap homemade kit to replace the expensive cornmercial kit on abi 310 capillary genetic analyzer. after studying the effect of temperature and urea denaturant on microsatellite analysis, 8 mol/l urea and 60 degrees c were selected for assessing accurately fragment size of microsatellite alleles. based on this method, 52 sporadic gastric cancers were screened, and msi and loh, at least one locus was observed in 15 of 52 (28.8%) patients. moreover, it is found that a statistically significant association exists between msi and loh and tumor-differentiated level. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | strand conformation polymorphism ; laser-induced fluorescence ; polymerase-chain-reaction ; tandem repeat loci ; k-ras gene ; repeated sequences ; x-chromosome ; mutation ; dna ; population |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000236927600016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97603]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China 3.Haebin Med Univ, Hosp Affiliated, Haerbin 150001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, XZ,Li, JH,Li, AY,et al. Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2006,834(1-2):122-127. |
| APA | Shi, XZ,Li, JH,Li, AY,Lv, S,&Xu, GW.(2006).Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit.journal of chromatography b-analytical technologies in the biomedical and life sciences,834(1-2),122-127. |
| MLA | Shi, XZ,et al."Simultaneous analysis of microsatellite instability and loss of heterozygosity by capillary electrophoresis with a homemade kit".journal of chromatography b-analytical technologies in the biomedical and life sciences 834.1-2(2006):122-127. |
入库方式： OAI收割
来源：大连化学物理研究所
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