Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)
文献类型：期刊论文
| 作者 | Yin, Peiyuan; Zhao, Xinjie; Li, Qiurong; Wang, Jiangshan; Li, Jieshou; Xu, Guowang |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 5期号:9页码:2135-2143 |
| 关键词 | metabonomics intestinal fistula UPLC/Q-TOF MS biomarker |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | ultraperformance liquid chromatography coupled with q-tof mass spectrometry (uplc/q-tof ms) is an effective and sensitive analytical tool. a uplc/q-tof ms-based metabonomics technique was employed to investigate sera from 40 patients with intestinal fistula and 17 healthy volunteers in an effort to find potential biomarkers of the disease and reveal their pathophysiological changes. after the uplc/q-tof analysis, the retention time and m/z data pair for each peak were detected. partial least squares discriminant analysis (pls-da) and coefficient of correlation analysis were used for marker selection and identification. according to the data, nine potential biomarkers were identified: glycochenodeoxycholic acid, glycodeoxycholic acid, taurochenodexycholic acid, taurodeoxycholic acid, and two kinds of lysophosphatidyl choline (c16: 0 and c18:2) were found with increased concentrations in the patients, and phenylalanine, tryptophan, and carnitine were found with decreased concentrations in the patients. the results suggested that a subclinical hepatic injury and abnormal metabolism of two essential amino acids ( phenylalanine and tryptophan), and a key compound of fatty acid synthesis and beta-oxidation (carnitine), occurred in the fistula patients. this work demonstrates the utility of metabonomics as a top-down systems biology tool for understanding clinical problems. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | biological-fluids ; bile-acids ; metabolism ; systems |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000240200700009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97615]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Gen Hosp, Nanjing Command PLA, Nanjing 210002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Peiyuan,Zhao, Xinjie,Li, Qiurong,et al. Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)[J]. journal of proteome research,2006,5(9):2135-2143. |
| APA | Yin, Peiyuan,Zhao, Xinjie,Li, Qiurong,Wang, Jiangshan,Li, Jieshou,&Xu, Guowang.(2006).Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS).journal of proteome research,5(9),2135-2143. |
| MLA | Yin, Peiyuan,et al."Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)".journal of proteome research 5.9(2006):2135-2143. |
入库方式： OAI收割
来源：大连化学物理研究所
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