Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection
文献类型：期刊论文
| 作者 | Pang, T; Bai, CM; Xu, YJ; Xu, GW; Yuan, ZY; Su, Y; Peng, LM |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:9页码:1281-1289 |
| 关键词 | solid-phase extraction liquid chromatography evaporative light scattering tobacco leaf sugars |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | a reversed phase liquid chromatography-evaporative light scattering detection, combined with solid phase extraction method was developed for the separation and determination of sugars in tobacco leaf. separation was performed with a hypersil nh2 analytical column and the mobile phase 75%:25% acetonitrile-water. in the range of 0.1-4 mg/ml, the method showed good linearity, with the correlation coefficients exceeding 0.99. the limits of detection of xylose, fructose, glucose, sucrose, maltose, and raffinose were 2, 2, 3, 2, 4, and 4 mu g/ml, respectively. the relative standard deviations of peak areas for these sugars were in the range of 2-3%. recoveries of fructose, glucose, and sucrose were between 96 and 117%. finally, determination of sugars in tobacco leaf, before and after bio-catalysis, was performed with this method. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; organic-acids ; gas-chromatography ; extracts |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000237497300006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97621]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Biochem & Cell Biol Inst, Shanghai Inst Biol Sci, Shanghai, Peoples R China 3.Chuxiong Cigarette Co, Hongta Grp, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pang, T,Bai, CM,Xu, YJ,et al. Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection[J]. journal of liquid chromatography & related technologies,2006,29(9):1281-1289. |
| APA | Pang, T.,Bai, CM.,Xu, YJ.,Xu, GW.,Yuan, ZY.,...&Peng, LM.(2006).Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection.journal of liquid chromatography & related technologies,29(9),1281-1289. |
| MLA | Pang, T,et al."Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection".journal of liquid chromatography & related technologies 29.9(2006):1281-1289. |
入库方式： OAI收割
来源：大连化学物理研究所
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