Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection
文献类型：期刊论文
| 作者 | Weng, QF; Xu, GW; Yuan, KL; Tang, P |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 835期号:1-2页码:55-61 |
| 关键词 | capillary electrophoresis field-amplified sample injection amperometric detection monoamines urine |
| 产权排序 | 1;1 |
| 通讯作者 | 许国旺 |
| 英文摘要 | a simple, rapid and low-cost method using capillary electrophoresis coupled with field-amplified sample stacking and electrochemical detection was developed for the separation and determination of monoamines. in this present work, a systematic study of the parameters (ph value and concentration of electrophoretic buffer, composition of sample solvent, injection voltage and time) affecting separation and on-line concentration of monoamines has been performed enabling the detection sensitivity of these monoamines to be improved by 5000 times compared with the conventional electrokinetic injection. this developed method was applied to the direct analysis of these monoamines in human urine without off-line sample preconcentration. due to the requirement for urine dilution to minimize the detrimental effects of high salt on analyte stacking, the real sensitivity improvement is about 50-fold when applying the optimized method to urine samples. in order to quantitate these monoamines accurately, internal standard calibration curves were constructed with standard monoamines in presence of salt with similar concentration as in human urine. in the method validation, the calibration curves were linear over a range of 1.0 x 10(-9) to 2.5 x 10(-8) mol/l for each monoamine and the limits of detection (signal to noise ratio of 3) for these monoamines were in the sub-nmol/l concentration range (6.0 x 10(-10) mol/l). (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; dynamic ph junction ; electrokinetic chromatography ; catecholamines ; column ; fluorescence ; metabolites ; injection ; serotonin ; dopamine |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000237369900008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97623]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Weng, QF,Xu, GW,Yuan, KL,et al. Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2006,835(1-2):55-61. |
| APA | Weng, QF,Xu, GW,Yuan, KL,&Tang, P.(2006).Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection.journal of chromatography b-analytical technologies in the biomedical and life sciences,835(1-2),55-61. |
| MLA | Weng, QF,et al."Determination of monoamines in urine by capillary electrophoresis with field-amplified sample stacking and amperometric detection".journal of chromatography b-analytical technologies in the biomedical and life sciences 835.1-2(2006):55-61. |
入库方式： OAI收割
来源：大连化学物理研究所
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