Application of Optical Protein-chip in Detecting Phage M13KO7
文献类型：期刊论文
| 作者 | Meng YH(孟永宏) ; Feng J(冯静); Jin G(靳刚)
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| 刊名 | CHINESE JOURNAL OF BIOTECHNOLOGY,2006,
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| 出版日期 | 2006 |
| 卷号 | 22期号:5页码:856-860 |
| ISSN号 | 1000-3061 |
| 其他题名 | 光学蛋白芯片技术在噬菌体M13KO7检测中的应用 |
| 合作状况 | 国内 |
| 中文摘要 | Abstract: Avidin layer was bound on the substrate surface of Silicon wafer modified with aldehyde. The interaction between avidin and biotin was adopted for the immobilization of mouse monoclonal biotin-anti-M13 (antibody GP3)-labeled biotin. The surface was incubated in a solution containing phage M13KO7, which was trapped by the antibody GP3 with the interaction between phage M13KO7 and antibody GP3, resulting in a variation of layer thickness that was detected by imaging ellipsometry. The results showed a saturated layer of antibody GP3 with a thickness about 6.9 nm on the surface of the silicon wafer. The specific interaction between phage M13KO7 and antibody GP3 resulted in a variation of layer thickness. The layer of phage M13KO7 bound with antibody GP3 was 17.5 nm in the concentration of 1.1×1010 pfu/mL. Each variation of the layer thickness corresponded to a concentration of phage M13KO7 in the range of 0.1×1010–2.5×1010 pfu/mL, with the sensitivity of 109 pfu/mL. Compared with other methods, the optical protein-chip, requiring only short measurement time, label free, is a quantitative test, and can be visualized. This study could be significant on the interactions between the antibody and the virus, showing potential in the early diagnosis of virosis. |
| 学科主题 | 力学 |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 公开日期 | 2007-06-15 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/17293]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Meng YH,Feng J,Jin G. Application of Optical Protein-chip in Detecting Phage M13KO7[J]. CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,2006,22(5):856-860. |
| APA | Meng YH,Feng J,&Jin G.(2006).Application of Optical Protein-chip in Detecting Phage M13KO7.CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,22(5),856-860. |
| MLA | Meng YH,et al."Application of Optical Protein-chip in Detecting Phage M13KO7".CHINESE JOURNAL OF BIOTECHNOLOGY,2006, 22.5(2006):856-860. |
入库方式： OAI收割
来源：力学研究所
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