The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market
文献类型：期刊论文
| 作者 | Wen, J ; Hu, CQ ; Zhang, L ; Luo, P ; Zhao, Z ; Fan, SG ; Su, T |
| 刊名 | FOOD CONTROL
 |
| 出版日期 | 2010 |
| 卷号 | 21期号:4页码:403-407 |
| ISSN号 | 0956-7135 |
| 关键词 | Sea cucumber PCR-RFLP FINS 16S rRNA gene Species identification |
| 通讯作者 | cqhu@scsio.ac.cn |
| 中文摘要 | Sea cucumbers are traditional, delicious and luxurious seafood in Asia. They are usually provided to the consumers in the marketing formats of dried or frozen products. At the commercial level, they are much different not only in flavor. but also in price. The processing treatment makes them difficult for species identification based on morphological characterization. In this study, two molecular methods are developed to identify six species belonging to family Stichopodidae in commercial products. The first one is PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism), which uses PCR amplification of a common 570 bp fragment of the 16S rRNA gene and follows codigestion with two restriction enzymes (Xmnl and Mwol). Another one is FINS (forensically informative nucleotide sequencing) of the 16S rRNA gene, and the key of the method is the phylogenetic analysis of 16S rRNA gene sequences. The PCR-RFLP methodology can be used to authenticate the species tested in the present study and allow us to detect a fraudulent labeling of frozen Apostichopus japonicus product. And the FINS methodology confirms that the species substituted for A. japonicus belongs to the genera Isostichopus. Therefore, two molecular tools really provide convenient, useful and academic techniques to authenticate the species of commercial sea cucumber products. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Food Science & Technology |
| 收录类别 | SCI |
| 原文出处 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000272752800008 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/4354]  |
| 专题 | 南海海洋研究所_中科院边缘海地质重点实验室 |
| 推荐引用方式 GB/T 7714 | Wen, J,Hu, CQ,Zhang, L,et al. The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market[J]. FOOD CONTROL,2010,21(4):403-407. |
| APA | Wen, J.,Hu, CQ.,Zhang, L.,Luo, P.,Zhao, Z.,...&Su, T.(2010).The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market.FOOD CONTROL,21(4),403-407. |
| MLA | Wen, J,et al."The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market".FOOD CONTROL 21.4(2010):403-407. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。