Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification
文献类型：期刊论文
| 作者 | Ren, CH ; Hu, CQ ; Luo, P ; Wang, QB |
| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 164期号:5页码:514-521 |
| 关键词 | Identification Vibrio vulnificus LAMP |
| ISSN号 | 0944-5013 |
| 通讯作者 | cqhu@scsio.ac.cn |
| 中文摘要 | Vibrio vulnificus is a serious bacterial. pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of V. vulnificus. The LAMP reaction was processed in a heat block at 65 degrees C for 60 min. The amplification products were detected by visual inspection using SYBR Green 1, as welt as by electrophoresis on agarose gets. Our results showed that the LAMP reaction was highly specific to V. vulnificus. This method was 10-fold more sensitive than conventional PCR. In conclusion, the LAMP assay was extremely rapid, simple, cost-effective, sensitive and specific for the rapid identification of V. vulnificus. (C) 2008 Elsevier GmbH. All rights reserved. |
| 学科主题 | Microbiology |
| 收录类别 | SCI |
| 原文出处 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| WOS记录号 | WOS:000271104500002 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5476]  |
| 专题 | 南海海洋研究所_广东省应用海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Ren, CH,Hu, CQ,Luo, P,et al. Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification[J]. MICROBIOLOGICAL RESEARCH,2009,164(5):514-521. |
| APA | Ren, CH,Hu, CQ,Luo, P,&Wang, QB.(2009).Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification.MICROBIOLOGICAL RESEARCH,164(5),514-521. |
| MLA | Ren, CH,et al."Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification".MICROBIOLOGICAL RESEARCH 164.5(2009):514-521. |
入库方式： OAI收割
来源：南海海洋研究所
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