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Up-regulation of PIK3CA promotes metastasis in gastric carcinoma
文献类型：期刊论文
| 作者 | Liu, JF ; Zhou, XK ; Chen, JH ; Yi, G ; Chen, HG ; Ba, MC ; Lin, SQ ; Qi, YC |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 16期号:39页码:4986-4991 |
| 关键词 | PIK3CA Phosphatidylinositol 3-kinase/Akt pathway Metastasis Gastric cancer Akt |
| ISSN号 | 1007-9327 |
| 通讯作者 | zxkstar@126.com |
| 中文摘要 | AIM: To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway. METHODS: mRNA and protein levels of PIK3CA were assessed, respectively, by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa, primary foci and lymph node and distant metastasis of gastric cancer. Akt and phosphorylated Akt protein were also examined by Western blotting in these tissues, in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling pathway. RESULTS: PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher, respectively, than that in the corresponding normal gastric mucosa and primary gastric cancer tissues (P < 0.05), while no statistical significance was found compared with distant metastasis. Immunohistochemically, PIK3CA protein expression was discovered in 7 (35%) specimens of 20 primary foci vs 10 (67%) of 15 of lymph node metastasis or 11(61%) of 18 of distant metastasis (35% vs 67%, P = 0.015; 35% vs 61%, P = 0.044). With the increased level of PIK3CA expression, the total Akt protein expression remained almost unchanged, but p-Akt protein was upregulated markedly. CONCLUSION: Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric cancer. Up-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling. (C) 2010 Baishideng. All rights reserved. |
| 学科主题 | Gastroenterology & Hepatology |
| 收录类别 | SCI |
| 原文出处 | W J G PRESS |
| WOS记录号 | WOS:000283396600015 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5480]  |
| 专题 | 南海海洋研究所_广东省应用海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, JF,Zhou, XK,Chen, JH,et al. Up-regulation of PIK3CA promotes metastasis in gastric carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(39):4986-4991. |
| APA | Liu, JF.,Zhou, XK.,Chen, JH.,Yi, G.,Chen, HG.,...&Qi, YC.(2010).Up-regulation of PIK3CA promotes metastasis in gastric carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,16(39),4986-4991. |
| MLA | Liu, JF,et al."Up-regulation of PIK3CA promotes metastasis in gastric carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 16.39(2010):4986-4991. |
入库方式： OAI收割
来源：南海海洋研究所
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