The elastic mechanical parameters of initial and pre-twisted copper wires were investigated with the aid of a self-developed micro-torsion tester. It was found that the apparent elastic shear modulus and the apparent Young tensile modulus of the copper wires decrease by 13.3 and 8.4% after pre-twist deformation, respectively. In addition, the indentation modulus also decreases as the deformation is increased. When the deformation is more than 30%, the indentation modulus of pre-twisted copper wires is smaller than that of initial copper wires. Microstructural analyses show that the elastic modulus decreases with the proportion of faults.