中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR

文献类型:会议论文

作者Chen, W ; Song, LR ; Ou, DY ; Gan, NQ
出版日期2005
会议名称6th International Conference on Toxic Cyanobacteria
会议日期JUN 21-27, 2004
会议地点Bergen, NORWAY
关键词Daphnia magna microcystin chronic toxicities ultrastructure enzyme
页码323-330
通讯作者Song, LR, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要In the current study, the toxicological mechanisms of microcystin-LR and its disadvantageous effects on Daphnia magna were examined. Survival rate, number of newborn, activity of several important enzymes [glutathione S-transferase (GST), lactate dehydrogenase (LDH), phosphatases, and glutathione], accumulated microcystins, and ultrastructural changes in different organs of Daphnia were monitored over the course of 21-day chronic tests. The results indicated that low concentrations of dissolved microcystin had no harmful effect on Daphnia. On the contrary, stimulatory effects were detected. In the presence of toxin at high dosage and for long-term exposure, GST and glutathione levels decreased significantly. The decreased enzyme activity in the antioxidant system probably was caused by detoxification reactions with toxins. And these processes of detoxification at the beginning of chronic tests may enable phosphatases in Daphnia magna to withstand inhibition by the toxins. At the same time, we also found that the LDH activity in test animals increased with exposure to microcystin-LR, indicating that adverse effects occurred in Daphnia. With microcystin given at a higher dosage or for a longer exposure, the effect on Daphnia magna was fatal. In the meantime, microcystin began to accumulate in Daphnia magna, and phosphatase activity started to be inhibited. From the ultrastructure results of cells in D. magna, we obtained new information: the alimentary canal may be the target organ affected by exposure of microcystins to D. magna. The results of the current study also suggested that the oxidative damage and PPI (protein phosphatase inhibition) mechanisms of vertebrates also are adapted to Daphnia. (c) 2005 Wiley Periodicals, Inc.
收录类别SCI
会议录ENVIRONMENTAL TOXICOLOGY
语种英语
WOS记录号WOS:000229897500016
源URL[http://ir.ihb.ac.cn/handle/342005/11795]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_会议论文
推荐引用方式
GB/T 7714
Chen, W,Song, LR,Ou, DY,et al. Chronic toxicity and responses of several important enzymes in Daphnia magna on exposure to sublethal microcystin-LR[C]. 见:6th International Conference on Toxic Cyanobacteria. Bergen, NORWAY. JUN 21-27, 2004.

入库方式: OAI收割

来源:水生生物研究所

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