GENE-TRANSFER VIA ELECTROPORATION IN FISH
文献类型：会议论文
| 作者 | XIE, YF ; LIU, D ; ZOU, J ; LI, GH ; ZHU, ZY |
| 出版日期 | 1993 |
| 会议名称 | 4TH INTERNATIONAL SYMP ON GENETICS IN AQUACULTURE |
| 会议日期 | APR 29-MAY 03, 1991 |
| 会议地点 | WUHAN, PEOPLES R CHINA |
| 关键词 | TRANSGENIC FISH EGGS |
| 页码 | 207-213 |
| 通讯作者 | XIE, YF, CHINESE ACAD SCI,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA |
| 中文摘要 | A fusion human growth hormone gene driven by the mouse metallothionein-1 promoter was successfully introduced into the fertilized eggs of loach and red crucian carp using a capacitor-based electroporator. The results of DNA hybridization showed that the transfer efficiency and the transgene copy number per genome were positively correlated to the voltage, duration and capacity of the pulse. Under optimum conditions, a transfer efficiency of up to 62.5% was achieved. Some fish arising from embryos treated in this manner exhibited over 100 copies of the transgene integrated into their genomes. |
| 收录类别 | SCI |
| 会议主办者 | CHINESE ACAD SCI, WUHAN BRANCH, HUBEI PROVINCIAL COMM SCI & TECHNOL, PEOPLES GOVT WUHAN, CHINESE ACAD SCI, INST HYDROBIOL, INT CULTURE EXCHANGE CTR, HUBEI BRANCH, YANTZE RIVER ACAD ECOL, PROFESSOR ZHUFA WU |
| 会议录 | AQUACULTURE
 |
| 语种 | 英语 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/11875]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_会议论文 |
| 推荐引用方式 GB/T 7714 | XIE, YF,LIU, D,ZOU, J,et al. GENE-TRANSFER VIA ELECTROPORATION IN FISH[C]. 见:4TH INTERNATIONAL SYMP ON GENETICS IN AQUACULTURE. WUHAN, PEOPLES R CHINA. APR 29-MAY 03, 1991. |
入库方式： OAI收割
来源：水生生物研究所
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