Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish
文献类型：期刊论文
| 作者 | Mo, Saijun1,2; Wang, Lu1,2; Li, Qing1,2; Li, Jie1,2; Li, Yuanyuan1,2; Thannickal, Victor J.3; Cui, Zongbin1,2 |
| 刊名 | DEVELOPMENTAL BIOLOGY
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 344期号:1页码:210-223 |
| 关键词 | Caveolin-1 beta-catenin Dorsoventral patterning Wnt signaling Zebrafish |
| ISSN号 | 0012-1606 |
| 通讯作者 | Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, 7 S Donghu Rd, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal beta-catenin and how Cav-1 functions in zebrafish embryogenesis Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish Both Win and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1 alpha or -1 beta resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes Cav-1 overexpression disrupts the nuclear translocation of beta-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of beta-catenin This reciprocal interaction is necessary for the ventralizing activity of Cav-1 We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active beta-catenin (C) 2010 Published by Elsevier Inc |
| 英文摘要 | Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal beta-catenin and how Cav-1 functions in zebrafish embryogenesis Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish Both Win and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1 alpha or -1 beta resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes Cav-1 overexpression disrupts the nuclear translocation of beta-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of beta-catenin This reciprocal interaction is necessary for the ventralizing activity of Cav-1 We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active beta-catenin (C) 2010 Published by Elsevier Inc |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Developmental Biology |
| 类目[WOS] | Developmental Biology |
| 研究领域[WOS] | Developmental Biology |
| 关键词[WOS] | WNT SIGNALING PATHWAY ; AXIS FORMATION ; IN-VIVO ; FUNCTIONAL INTERACTION ; INDUCED ANTAGONIST ; PROTEIN-COMPONENT ; MEMBRANE DOMAINS ; XENOPUS EMBRYOS ; EXPRESSION ; ORGANIZER |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2009CB941200]; National Natural Science Foundation of China [30671602, 30871442]; National Institute of Health, USA [R01 HL067967] |
| 语种 | 英语 |
| WOS记录号 | WOS:000280426400019 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13745]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China 3.Univ Alabama, Div Pulm Allergy & Crit Care Med, Birmingham, AL USA |
| 推荐引用方式 GB/T 7714 | Mo, Saijun,Wang, Lu,Li, Qing,et al. Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish[J]. DEVELOPMENTAL BIOLOGY,2010,344(1):210-223. |
| APA | Mo, Saijun.,Wang, Lu.,Li, Qing.,Li, Jie.,Li, Yuanyuan.,...&Cui, Zongbin.(2010).Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish.DEVELOPMENTAL BIOLOGY,344(1),210-223. |
| MLA | Mo, Saijun,et al."Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish".DEVELOPMENTAL BIOLOGY 344.1(2010):210-223. |
入库方式： OAI收割
来源：水生生物研究所
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