Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1
文献类型：期刊论文
| 作者 | Li Xi; Nie Fen; Yin Zhan; He JiangYan |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 54期号:2页码:159-165 |
| 关键词 | Follistatin1 myostatin muscle growth transgenic zebrafish |
| ISSN号 | 1674-7305 |
| 通讯作者 | He, JY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; jyhe@ihb.ac.cn |
| 中文摘要 | Myostatin is a member of the transforming growth factor-beta (TGF-beta) super-family and functions as a negative regulator of muscle growth. Binding of the specific receptor, Activin receptor IIB (Act RIIB), with myostatin or other related TGF-beta members, could be inhibited by the activin-binding protein follistatin (Fst) in mammals. Overexpressing Fst in mouse skeletal muscle leads to muscle hypertrophy and hyperplasia. To determine if Fst has similar roles in fish, we generated transgenic zebrafish expressing high levels of zebrafish Fst1 using the promoter of the zebrafish skeletal muscle-specific gene, myosin, light polypeptide 2, skeletal muscle (Mylz2). Independent transgenic zebrafish lines exhibited elevated expression levels of myogenic regulatory genes MyoD and Pax7 in muscle cells. Adult Fst1 overexpressing transgenic zebrafish exhibited a slight body weight increase. The high level of Fst1 expression dramatically increased myofiber numbers in skeletal muscle, without significantly changing the fiber size. Our findings suggest that Fst1 overexpression can promote zebrafish muscle growth by enhancing myofiber hyperplasia. |
| 英文摘要 | Myostatin is a member of the transforming growth factor-beta (TGF-beta) super-family and functions as a negative regulator of muscle growth. Binding of the specific receptor, Activin receptor IIB (Act RIIB), with myostatin or other related TGF-beta members, could be inhibited by the activin-binding protein follistatin (Fst) in mammals. Overexpressing Fst in mouse skeletal muscle leads to muscle hypertrophy and hyperplasia. To determine if Fst has similar roles in fish, we generated transgenic zebrafish expressing high levels of zebrafish Fst1 using the promoter of the zebrafish skeletal muscle-specific gene, myosin, light polypeptide 2, skeletal muscle (Mylz2). Independent transgenic zebrafish lines exhibited elevated expression levels of myogenic regulatory genes MyoD and Pax7 in muscle cells. Adult Fst1 overexpressing transgenic zebrafish exhibited a slight body weight increase. The high level of Fst1 expression dramatically increased myofiber numbers in skeletal muscle, without significantly changing the fiber size. Our findings suggest that Fst1 overexpression can promote zebrafish muscle growth by enhancing myofiber hyperplasia. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biology |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | DANIO-RERIO ; MYOSTATIN ; GENE ; GROWTH ; OVEREXPRESSION ; TELEOSTS ; DEFECTS ; MICE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30671600]; National Basic Research Program of China [2010CB126302, 2009CB118701] |
| 语种 | 英语 |
| WOS记录号 | WOS:000287702200008 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15520]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Xi,Nie Fen,Yin Zhan,et al. Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(2):159-165. |
| APA | Li Xi,Nie Fen,Yin Zhan,&He JiangYan.(2011).Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1.SCIENCE CHINA-LIFE SCIENCES,54(2),159-165. |
| MLA | Li Xi,et al."Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1".SCIENCE CHINA-LIFE SCIENCES 54.2(2011):159-165. |
入库方式： OAI收割
来源：水生生物研究所
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