Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells
文献类型：期刊论文
| 作者 | Ge, Feng1; Zhang, Liang2; Tao, Sheng-Ce3; Kitazato, Kaio4; Zhang, Zhi-Ping5; Zhang, Xian-En5; Bi, Li-Jun6 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 10期号:2页码:845-855 |
| ISSN号 | 1535-3893 |
| 关键词 | Multiple myeloma (MM) tumor reversion stable isotope labeling by amino acids in cell culture (SILAC) signal transducer and activator of transcription 3 (STAT3) |
| 通讯作者 | Ge, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; gefeng@ihb.ac.cn, blj@sun5.ibp.ac.cn |
| 中文摘要 | Tumor reversion is defined as the process by which cancer cells lose their malignant phenotype. However, relatively little is known about the cellular proteome changes that occur during the reversion process. A biological model of multiple myelonna (MM) reversion was established by using the H-1 parvovirus as a tool to select for revertant cells from MM cells. Isolated revertant cells displayed a strongly suppressed malignant phenotype both in vitro and in vivo. To explore possible mechanisms of MM reversion, the protein profiles of the revertant and parental MM cells were compared using a quantitative proteomic strategy termed SILAC-MS. Our results revealed that 379 proteins were either activated or inhibited during the reversion process, with a much greater proportion of the proteins, including STAT3, TCTP, CDC2, BAG2, and PCNA, being inhibited. Of these, STAT3, which is significantly down regulated, was selected for further functional studies. Inhibition of STAT3 expression by RNA interference resulted in suppression of the malignant phenotype and concomitant down regulation of TCTP expression, suggesting that myeloma reversion operates, at least in part, through inhibition of STAT3. Our results provide novel insights into the mechanisms of tumor reversion and suggest new alternative approaches for MM treatment. |
| 英文摘要 | Tumor reversion is defined as the process by which cancer cells lose their malignant phenotype. However, relatively little is known about the cellular proteome changes that occur during the reversion process. A biological model of multiple myelonna (MM) reversion was established by using the H-1 parvovirus as a tool to select for revertant cells from MM cells. Isolated revertant cells displayed a strongly suppressed malignant phenotype both in vitro and in vivo. To explore possible mechanisms of MM reversion, the protein profiles of the revertant and parental MM cells were compared using a quantitative proteomic strategy termed SILAC-MS. Our results revealed that 379 proteins were either activated or inhibited during the reversion process, with a much greater proportion of the proteins, including STAT3, TCTP, CDC2, BAG2, and PCNA, being inhibited. Of these, STAT3, which is significantly down regulated, was selected for further functional studies. Inhibition of STAT3 expression by RNA interference resulted in suppression of the malignant phenotype and concomitant down regulation of TCTP expression, suggesting that myeloma reversion operates, at least in part, through inhibition of STAT3. Our results provide novel insights into the mechanisms of tumor reversion and suggest new alternative approaches for MM treatment. |
| 学科主题 | Biochemical Research Methods |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HISTAMINE-RELEASING FACTOR ; ANTIAPOPTOTIC ACTIVITY ; MONOCLONAL GAMMOPATHY ; HUMAN BASOPHILS ; BONE-MARROW ; PROTEIN ; APOPTOSIS ; CANCER ; DIFFERENTIATION ; TCTP |
| 资助信息 | Chinese Academy of Sciences ; National Protein Research Fund [2009CB825400, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Program for New Century Excellent Talents in University [NCET-09-551]; Japan Society for the Promotion of Science (JSPS) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286868500044 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15522]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Singapore Hlth Res Facil, Div Res, Singapore 169611, Singapore 3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China 4.Nagasaki Univ, Dept Mol Microbiol & Immunol, Nagasaki 8528521, Japan 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 6.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Feng,Zhang, Liang,Tao, Sheng-Ce,et al. Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(2):845-855. |
| APA | Ge, Feng.,Zhang, Liang.,Tao, Sheng-Ce.,Kitazato, Kaio.,Zhang, Zhi-Ping.,...&Bi, Li-Jun.(2011).Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells.JOURNAL OF PROTEOME RESEARCH,10(2),845-855. |
| MLA | Ge, Feng,et al."Quantitative Proteomic Analysis of Tumor Reversion in Multiple Myeloma Cells".JOURNAL OF PROTEOME RESEARCH 10.2(2011):845-855. |
入库方式： OAI收割
来源：水生生物研究所
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