Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species
文献类型：期刊论文
| 作者 | Rao, Yu1,2; Zhong, Liqiao1,2; Liao, Tao1; Jin, Shiwei1; Wang, Yuzhen1; Song, Bo1; Li, Jia1,2; Zhang, Xiaohua1; Hemmingsen, Sean M.3; Xu, Ying1 |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2010-12-07 |
| 卷号 | 93期号:1页码:83-91 |
| 关键词 | Vitellogenin detection Cyprinid fish Phage display ScFv antibody Bioassay Binding affinity |
| ISSN号 | 0177-5103 |
| 通讯作者 | Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 So E Lake Rd, Wuhan 430072, Peoples R China ; hpdai@ihb.ac.cn |
| 中文摘要 | Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish. |
| 英文摘要 | Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | ZEBRAFISH DANIO-RERIO ; MINNOW PIMEPHALES-PROMELAS ; LINKED-IMMUNOSORBENT-ASSAY ; ESTROGENIC CHEMICALS ; GOBIOCYPRIS-RARUS ; CARP ; BIOMARKER ; IMMUNOASSAY ; ELISA ; PURIFICATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424] |
| 语种 | 英语 |
| WOS记录号 | WOS:000285305100008 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15530]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Natl Res Council Canada, Plant Biotechnol Inst, Saskatoon, SK S7N 0W9, Canada |
| 推荐引用方式 GB/T 7714 | Rao, Yu,Zhong, Liqiao,Liao, Tao,et al. Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species[J]. DISEASES OF AQUATIC ORGANISMS,2010,93(1):83-91. |
| APA | Rao, Yu.,Zhong, Liqiao.,Liao, Tao.,Jin, Shiwei.,Wang, Yuzhen.,...&Dai, Heping.(2010).Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.DISEASES OF AQUATIC ORGANISMS,93(1),83-91. |
| MLA | Rao, Yu,et al."Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species".DISEASES OF AQUATIC ORGANISMS 93.1(2010):83-91. |
入库方式： OAI收割
来源：水生生物研究所
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