MRP proteins as potential mediators of heavy metal resistance in zebrafish cells
文献类型：期刊论文
| 作者 | Long, Yong1; Li, Qing1; Wang, Youhui2; Cui, Zongbin1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 153期号:3页码:310-317 |
| 关键词 | Zebrafish MRP GSH GST Heavy metal Detoxification |
| ISSN号 | 1532-0456 |
| 通讯作者 | Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China ; zbcui@ihb.ac.cn |
| 中文摘要 | Acquired resistance of mammalian cells to heavy metals is closely relevant to enhanced expression of several multidrug resistance-associated proteins (MRP), but it remains unclear whether MRP proteins confer resistance to heavy metals in zebrafish. In this study, we obtained zebrafish (Danio rerio) fibroblast-like ZF4 cells with resistance to toxic heavy metals after chronic cadmium exposure and selection for 6 months. These cadmium-resistant cells (ZF4-Cd) were maintained in 5 mu M cadmium and displayed cross-resistance to cadmium, mercury, arsenite and arsenate. ZF4-Cd cells remained the resistance to heavy metals after protracted culture in cadmium-free medium. In comparison with ZF4-WT cells, ZF4-Cd cells exhibited accelerated rate of cadmium excretion, enhanced activity of MRP-like transport, elevated expression of abcc2, abcc4 and mt2 genes, and increased content of cellular GSH. Inhibition of MRP-like transport activity, GSH biosynthesis and GST activity significantly attenuated the resistance of ZF4-Cd cells to heavy metals. The results indicate that some of MRP transporters are involved in the efflux of heavy metals conjugated with cellular GSH and thus play crucial roles in heavy metal detoxification of zebrafish cells. (C) 2010 Elsevier Inc. All rights reserved. |
| 英文摘要 | Acquired resistance of mammalian cells to heavy metals is closely relevant to enhanced expression of several multidrug resistance-associated proteins (MRP), but it remains unclear whether MRP proteins confer resistance to heavy metals in zebrafish. In this study, we obtained zebrafish (Danio rerio) fibroblast-like ZF4 cells with resistance to toxic heavy metals after chronic cadmium exposure and selection for 6 months. These cadmium-resistant cells (ZF4-Cd) were maintained in 5 mu M cadmium and displayed cross-resistance to cadmium, mercury, arsenite and arsenate. ZF4-Cd cells remained the resistance to heavy metals after protracted culture in cadmium-free medium. In comparison with ZF4-WT cells, ZF4-Cd cells exhibited accelerated rate of cadmium excretion, enhanced activity of MRP-like transport, elevated expression of abcc2, abcc4 and mt2 genes, and increased content of cellular GSH. Inhibition of MRP-like transport activity, GSH biosynthesis and GST activity significantly attenuated the resistance of ZF4-Cd cells to heavy metals. The results indicate that some of MRP transporters are involved in the efflux of heavy metals conjugated with cellular GSH and thus play crucial roles in heavy metal detoxification of zebrafish cells. (C) 2010 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; P-GLYCOPROTEIN INHIBITORS ; RENAL EPITHELIAL-CELLS ; LUNG-TUMOR CELLS ; MULTIDRUG-RESISTANCE ; EFFLUX TRANSPORTERS ; CELLULAR-FUNCTIONS ; ARSENIC-RESISTANT ; CADMIUM TOXICITY ; GLUTATHIONE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30671602, 30871442] |
| 语种 | 英语 |
| WOS记录号 | WOS:000288298700006 |
| 公开日期 | 2011-06-30 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15678]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Yong,Li, Qing,Wang, Youhui,et al. MRP proteins as potential mediators of heavy metal resistance in zebrafish cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2011,153(3):310-317. |
| APA | Long, Yong,Li, Qing,Wang, Youhui,&Cui, Zongbin.(2011).MRP proteins as potential mediators of heavy metal resistance in zebrafish cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,153(3),310-317. |
| MLA | Long, Yong,et al."MRP proteins as potential mediators of heavy metal resistance in zebrafish cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 153.3(2011):310-317. |
入库方式： OAI收割
来源：水生生物研究所
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