中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

文献类型:期刊论文

作者Long, Yong1; Li, Qing1; Wang, Youhui2; Cui, Zongbin1
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
出版日期2011-04-01
卷号153期号:3页码:310-317
关键词Zebrafish MRP GSH GST Heavy metal Detoxification
ISSN号1532-0456
通讯作者Cui, ZB, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China ; zbcui@ihb.ac.cn
中文摘要Acquired resistance of mammalian cells to heavy metals is closely relevant to enhanced expression of several multidrug resistance-associated proteins (MRP), but it remains unclear whether MRP proteins confer resistance to heavy metals in zebrafish. In this study, we obtained zebrafish (Danio rerio) fibroblast-like ZF4 cells with resistance to toxic heavy metals after chronic cadmium exposure and selection for 6 months. These cadmium-resistant cells (ZF4-Cd) were maintained in 5 mu M cadmium and displayed cross-resistance to cadmium, mercury, arsenite and arsenate. ZF4-Cd cells remained the resistance to heavy metals after protracted culture in cadmium-free medium. In comparison with ZF4-WT cells, ZF4-Cd cells exhibited accelerated rate of cadmium excretion, enhanced activity of MRP-like transport, elevated expression of abcc2, abcc4 and mt2 genes, and increased content of cellular GSH. Inhibition of MRP-like transport activity, GSH biosynthesis and GST activity significantly attenuated the resistance of ZF4-Cd cells to heavy metals. The results indicate that some of MRP transporters are involved in the efflux of heavy metals conjugated with cellular GSH and thus play crucial roles in heavy metal detoxification of zebrafish cells. (C) 2010 Elsevier Inc. All rights reserved.
英文摘要Acquired resistance of mammalian cells to heavy metals is closely relevant to enhanced expression of several multidrug resistance-associated proteins (MRP), but it remains unclear whether MRP proteins confer resistance to heavy metals in zebrafish. In this study, we obtained zebrafish (Danio rerio) fibroblast-like ZF4 cells with resistance to toxic heavy metals after chronic cadmium exposure and selection for 6 months. These cadmium-resistant cells (ZF4-Cd) were maintained in 5 mu M cadmium and displayed cross-resistance to cadmium, mercury, arsenite and arsenate. ZF4-Cd cells remained the resistance to heavy metals after protracted culture in cadmium-free medium. In comparison with ZF4-WT cells, ZF4-Cd cells exhibited accelerated rate of cadmium excretion, enhanced activity of MRP-like transport, elevated expression of abcc2, abcc4 and mt2 genes, and increased content of cellular GSH. Inhibition of MRP-like transport activity, GSH biosynthesis and GST activity significantly attenuated the resistance of ZF4-Cd cells to heavy metals. The results indicate that some of MRP transporters are involved in the efflux of heavy metals conjugated with cellular GSH and thus play crucial roles in heavy metal detoxification of zebrafish cells. (C) 2010 Elsevier Inc. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology
类目[WOS]Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
研究领域[WOS]Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
关键词[WOS]TROUT ONCORHYNCHUS-MYKISS ; P-GLYCOPROTEIN INHIBITORS ; RENAL EPITHELIAL-CELLS ; LUNG-TUMOR CELLS ; MULTIDRUG-RESISTANCE ; EFFLUX TRANSPORTERS ; CELLULAR-FUNCTIONS ; ARSENIC-RESISTANT ; CADMIUM TOXICITY ; GLUTATHIONE
收录类别SCI
资助信息National Natural Science Foundation of China [30671602, 30871442]
语种英语
WOS记录号WOS:000288298700006
公开日期2011-06-30
源URL[http://ir.ihb.ac.cn/handle/342005/15678]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Long, Yong,Li, Qing,Wang, Youhui,et al. MRP proteins as potential mediators of heavy metal resistance in zebrafish cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2011,153(3):310-317.
APA Long, Yong,Li, Qing,Wang, Youhui,&Cui, Zongbin.(2011).MRP proteins as potential mediators of heavy metal resistance in zebrafish cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,153(3),310-317.
MLA Long, Yong,et al."MRP proteins as potential mediators of heavy metal resistance in zebrafish cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 153.3(2011):310-317.

入库方式: OAI收割

来源:水生生物研究所

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