A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater
文献类型：期刊论文
| 作者 | Tu, Xinhai2; Xiao, Bangding1; Xiong, Jian2; Chen, Xudong |
| 刊名 | TALANTA
 |
| 出版日期 | 2010-08-15 |
| 卷号 | 82期号:3页码:976-983 |
| 关键词 | Nitrate Nitrite Microplate Water analysis |
| ISSN号 | 0039-9140 |
| 通讯作者 | Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L-1 for NO2--N and 0.02 to 1.50 mg L-1 for NO3--N. The detection limits for nitrite and nitrate were 2 and 4 mu g L-1, respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved. |
| 英文摘要 | A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L(-1) for NO(2)(-)-N and 0.02 to 1.50 mg L(-1) for NO(3)(-)-N. The detection limits for nitrite and nitrate were 2 and 4 mu g L(-1), respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry, Analytical |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | FLOW-INJECTION ANALYSIS ; SPECTROPHOTOMETRIC DETERMINATION ; CADMIUM COLUMN ; COPPER-CADMIUM ; REDUCTION ; PHOSPHATE ; SYSTEM ; SOIL |
| 收录类别 | SCI |
| 资助信息 | Special Program Water Pollution Control in China [2008ZX07102-005]; Science Foundation of NSFC-Yunnan Province [U0833604]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426] |
| 语种 | 英语 |
| WOS记录号 | WOS:000281177100017 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13687]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tu, Xinhai,Xiao, Bangding,Xiong, Jian,et al. A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater[J]. TALANTA,2010,82(3):976-983. |
| APA | Tu, Xinhai,Xiao, Bangding,Xiong, Jian,&Chen, Xudong.(2010).A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater.TALANTA,82(3),976-983. |
| MLA | Tu, Xinhai,et al."A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater".TALANTA 82.3(2010):976-983. |
入库方式： OAI收割
来源：水生生物研究所
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