Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)
文献类型：期刊论文
| 作者 | Jin, Shiwei1,2; Yang, Fangxing1; Hui, Yang1; Xu, Ying1; Lu, Yuanan1,3; Liu, Jiankang1 |
| 刊名 | TOXICOLOGY IN VITRO
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 24期号:4页码:1190-1196 |
| 关键词 | 2 Decabrominated diphenyl ether RTG-2 cells Cytotoxicity Apoptosis Reactive oxygen species 2 ' 4 4 '-Tetrabromodiphenyl ether |
| ISSN号 | 0887-2333 |
| 通讯作者 | Xu, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Recently, the environmental residues of polybrominated diphenyl ethers (PBDEs) have markedly increased. In particular, the levels of certain PBDE congeners in fish have raised concern regarding potential risks associated with dietary PBDEs exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro fish cell models. In this study, the cytotoxic effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209) on RTG-2 cells were investigated. RTG-2 cells were incubated with different concentrations of BDE-47 and BDE-209 (1-100 mu M) for 72 h, and a set of bioassays were conducted to measure: cell viability (evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and neutral red (NR) uptake), lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS) formation and cell apoptosis. The results showed that BDE-47 and BDE-209 inhibited the cells viability, increased LDH leakage, and induced cell apoptosis in time and concentration-dependent manner. All significant effects were observed at concentrations of 12.5 mu M and above for BDE-47 and 25 mu M and above for BDE-209 (P < 0.05). At the concentration of 100 mu M BDE-47 and BDE-209, the cell viability of the exposed cells dropped to about 40% and 50% of the control, and the apoptotic rates were 52.6% and 34.6%, respectively. After 12 h exposure, a concentration-dependent increases of BDE-47 and BDE-209 (12.5-100 mu M) in ROS formation were observed. Collectively, the results of cell viability. LDH leakage, cell apoptosis and ROS formation demonstrated that the toxic mechanism of PBDEs on RTG-2 might be mediated by oxidative stress. (C) 2010 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Recently, the environmental residues of polybrominated diphenyl ethers (PBDEs) have markedly increased. In particular, the levels of certain PBDE congeners in fish have raised concern regarding potential risks associated with dietary PBDEs exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro fish cell models. In this study, the cytotoxic effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209) on RTG-2 cells were investigated. RTG-2 cells were incubated with different concentrations of BDE-47 and BDE-209 (1-100 mu M) for 72 h, and a set of bioassays were conducted to measure: cell viability (evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and neutral red (NR) uptake), lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS) formation and cell apoptosis. The results showed that BDE-47 and BDE-209 inhibited the cells viability, increased LDH leakage, and induced cell apoptosis in time and concentration-dependent manner. All significant effects were observed at concentrations of 12.5 mu M and above for BDE-47 and 25 mu M and above for BDE-209 (P < 0.05). At the concentration of 100 mu M BDE-47 and BDE-209, the cell viability of the exposed cells dropped to about 40% and 50% of the control, and the apoptotic rates were 52.6% and 34.6%, respectively. After 12 h exposure, a concentration-dependent increases of BDE-47 and BDE-209 (12.5-100 mu M) in ROS formation were observed. Collectively, the results of cell viability. LDH leakage, cell apoptosis and ROS formation demonstrated that the toxic mechanism of PBDEs on RTG-2 might be mediated by oxidative stress. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Toxicology |
| 类目[WOS] | Toxicology |
| 研究领域[WOS] | Toxicology |
| 关键词[WOS] | BROMINATED FLAME RETARDANTS ; IN-VITRO ; DEVELOPMENTAL EXPOSURE ; POLYCHLORINATED-BIPHENYLS ; OXIDATIVE STRESS ; SEDIMENTS ; PBDES ; NEUROTOXICITY ; ENVIRONMENT ; TOXICITY |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] |
| 语种 | 英语 |
| WOS记录号 | WOS:000279124900019 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13693]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China 3.Univ Hawaii, Dept Publ Hlth Sci, John A Burns Sch Med, Honolulu, HI 96822 USA |
| 推荐引用方式 GB/T 7714 | Jin, Shiwei,Yang, Fangxing,Hui, Yang,et al. Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)[J]. TOXICOLOGY IN VITRO,2010,24(4):1190-1196. |
| APA | Jin, Shiwei,Yang, Fangxing,Hui, Yang,Xu, Ying,Lu, Yuanan,&Liu, Jiankang.(2010).Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209).TOXICOLOGY IN VITRO,24(4),1190-1196. |
| MLA | Jin, Shiwei,et al."Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)".TOXICOLOGY IN VITRO 24.4(2010):1190-1196. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。