Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53
文献类型：期刊论文
| 作者 | Su, F.1; Pascal, L. E.1; Xiao, W.2; Wang, Z.1,3 |
| 刊名 | ONCOGENE
 |
| 出版日期 | 2010-01-21 |
| 卷号 | 29期号:3页码:421-431 |
| 关键词 | U19/EAF2 prostate cancer tumor suppressor TSP-1 p53 |
| ISSN号 | 0950-9232 |
| 通讯作者 | Wang, Z, Univ Pittsburgh, Sch Med, Inst Canc, Dept Urol, 5200 Ctr Ave,Suite G40, Pittsburgh, PA 15232 USA |
| 中文摘要 | Inactivation of U19/EAF2 has been shown previously to lead to tumorigenesis in multiple organs; however, the mechanism of U19/EAF2 tumor suppression remains unclear. In this paper, we report that the expression of an anti-angiogenic protein, thrombospondin-1 (TSP-1) is down-regulated in the prostate and liver of U19/EAF2 knockout mouse. The U19/EAF2 knockout liver displayed increased CD31-positive blood vessels, suggesting that the TSP-1 down-regulation can contribute to increased angiogenesis. TSP-1 is reported to be a p53-target gene and p53 is a known binding partner of ELL, which binds to U19/EAF2. Here, we show that U19/EAF2 can co-localize and co-immunoprecipitate with p53 in transfected cells. In a TSP-1 promoter-driven luciferase reporter assay, p53 transfection suppressed the TSP-1 promoter activity and U19/EAF2 co-transfection blocked the p53 suppression of TSP-1 promoter. However, U19/EAF2 transfection alone had little or no effect on the TSP-1 promoter. The above observations together suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. Oncogene (2010) 29, 421-431; doi:10.1038/onc.2009.326; published online 12 October 2009 |
| 英文摘要 | Inactivation of U19/EAF2 has been shown previously to lead to tumorigenesis in multiple organs; however, the mechanism of U19/EAF2 tumor suppression remains unclear. In this paper, we report that the expression of an anti-angiogenic protein, thrombospondin-1 (TSP-1) is down-regulated in the prostate and liver of U19/EAF2 knockout mouse. The U19/EAF2 knockout liver displayed increased CD31-positive blood vessels, suggesting that the TSP-1 down-regulation can contribute to increased angiogenesis. TSP-1 is reported to be a p53-target gene and p53 is a known binding partner of ELL, which binds to U19/EAF2. Here, we show that U19/EAF2 can co-localize and co-immunoprecipitate with p53 in transfected cells. In a TSP-1 promoter-driven luciferase reporter assay, p53 transfection suppressed the TSP-1 promoter activity and U19/EAF2 co-transfection blocked the p53 suppression of TSP-1 promoter. However, U19/EAF2 transfection alone had little or no effect on the TSP-1 promoter. The above observations together suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. Oncogene (2010) 29, 421-431; doi:10.1038/onc.2009.326; published online 12 October 2009 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
| 关键词[WOS] | HIPPEL-LINDAU PROTEIN ; ELONGATION-FACTOR ELL ; PROSTATE-CANCER ; GENE ; ANGIOGENESIS ; GROWTH ; CELLS ; CARCINOMA ; APOPTOSIS ; TRANSACTIVATION |
| 收录类别 | SCI |
| 资助信息 | NIH [R01 CA 120386, R37 DK51993]; Prostate Cancer Specialized Program of Research Excellence (SPORE) [P50 CA90386] |
| 语种 | 英语 |
| WOS记录号 | WOS:000273793200011 |
| 公开日期 | 2011-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13860]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Univ Pittsburgh, Sch Med, Inst Canc, Dept Urol, Pittsburgh, PA 15232 USA 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China 3.Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15232 USA |
| 推荐引用方式 GB/T 7714 | Su, F.,Pascal, L. E.,Xiao, W.,et al. Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53[J]. ONCOGENE,2010,29(3):421-431. |
| APA | Su, F.,Pascal, L. E.,Xiao, W.,&Wang, Z..(2010).Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53.ONCOGENE,29(3),421-431. |
| MLA | Su, F.,et al."Tumor suppressor U19/EAF2 regulates thrombospondin-1 expression via p53".ONCOGENE 29.3(2010):421-431. |
入库方式： OAI收割
来源：水生生物研究所
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