Isolation and characterization of microsatellite loci in Rhinogobio ventralis
文献类型:期刊论文
作者 | Xu, Nian1,2,3,4; Shi, Fang1,2,3,4; Xiong, Meihua1,2; Que, Yanfu1,2; Yang, Zhong1,2; Zhu, Bin1,2; Chang, Jianbo1,2 |
刊名 | CONSERVATION GENETICS RESOURCES
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出版日期 | 2010-09-01 |
卷号 | 2页码:1-3 |
关键词 | Rhinogobio ventralis Microsatellites Polymorphic loci Population genetics |
ISSN号 | 1877-7252 |
通讯作者 | Chang, JB, Minist Water Resources, Inst Hydroecol, 578th Xiongchu Ave, Wuhan 430079, Hubei, Peoples R China ; jbchang@mail.ihe.ac.cn |
中文摘要 | From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species. |
英文摘要 | From (AAAG)(n) enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biodiversity Conservation ; Genetics & Heredity |
研究领域[WOS] | Biodiversity & Conservation ; Genetics & Heredity |
关键词[WOS] | DNA |
收录类别 | SCI |
资助信息 | hydro-biodiversity monitoring at Pengshui hydropower station on Wujiang River [PS/HB011-2006] |
语种 | 英语 |
WOS记录号 | WOS:000290413000001 |
公开日期 | 2011-06-29 |
源URL | [http://ir.ihb.ac.cn/handle/342005/15576] ![]() |
专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
作者单位 | 1.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan 430079, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Nian,Shi, Fang,Xiong, Meihua,et al. Isolation and characterization of microsatellite loci in Rhinogobio ventralis[J]. CONSERVATION GENETICS RESOURCES,2010,2:1-3. |
APA | Xu, Nian.,Shi, Fang.,Xiong, Meihua.,Que, Yanfu.,Yang, Zhong.,...&Chang, Jianbo.(2010).Isolation and characterization of microsatellite loci in Rhinogobio ventralis.CONSERVATION GENETICS RESOURCES,2,1-3. |
MLA | Xu, Nian,et al."Isolation and characterization of microsatellite loci in Rhinogobio ventralis".CONSERVATION GENETICS RESOURCES 2(2010):1-3. |
入库方式: OAI收割
来源:水生生物研究所
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