Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)
文献类型：期刊论文
| 作者 | Li, Guangyu; Xie, Ping; Li, Huiying; Chen, Jun; Hao, Le; Xiong, Qian |
| 刊名 | JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 24期号:4页码:250-259 |
| 关键词 | Glutathione S-Transferases Tissue Distribution Real-Time PCR Gene Expression Aristichthys nobilis |
| ISSN号 | 1095-6670 |
| 通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | The expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle; the other two isoforms, GST alpha and rho, were also highly expressed in liver, kidney, intestine, spleen, heart, and brain of bighead carp. All these results strongly imply an important role of these GST isoforms in detoxification of ingested xenobiotics. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:250-259, 2010; View this article online at wileyonlinelibrary.com. DOI10:1002/jbt.20333 |
| 英文摘要 | The expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle; the other two isoforms, GST alpha and rho, were also highly expressed in liver, kidney, intestine, spleen, heart, and brain of bighead carp. All these results strongly imply an important role of these GST isoforms in detoxification of ingested xenobiotics. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:250-259, 2010; View this article online at wileyonlinelibrary.com. DOI10:1002/jbt.20333 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Toxicology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Toxicology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Toxicology |
| 关键词[WOS] | PLAICE PLEURONECTES-PLATESSA ; OXIDATIVE STRESS ; CLASS-KAPPA ; FISH ; DETOXIFICATION ; BIOMARKERS ; MOUSE ; SUSCEPTIBILITY ; IDENTIFICATION ; ISOENZYMES |
| 收录类别 | SCI |
| 资助信息 | Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] |
| 语种 | 英语 |
| WOS记录号 | WOS:000281455800007 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13522]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guangyu,Xie, Ping,Li, Huiying,et al. Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2010,24(4):250-259. |
| APA | Li, Guangyu,Xie, Ping,Li, Huiying,Chen, Jun,Hao, Le,&Xiong, Qian.(2010).Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis).JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,24(4),250-259. |
| MLA | Li, Guangyu,et al."Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 24.4(2010):250-259. |
入库方式： OAI收割
来源：水生生物研究所
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