Microcystin Extracts Induce Ultrastructural Damage and Biochemical Disturbance in Male Rabbit Testis
文献类型：期刊论文
| 作者 | Liu, Ying; Xie, Ping; Qiu, Tong; Li, Hui-Ying; Li, Guang-Yu; Hao, Le; Xiong, Qian |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 25期号:1页码:9-17 |
| 关键词 | microcystin extracts rabbit testis ultrastructure biochemical index |
| ISSN号 | 1520-4081 |
| 通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Beijing 100864, Peoples R China |
| 中文摘要 | In the present research, the changes of ultrastructures and biochemical index in rabbit testis were examined after i.p. injection with 12.5 mu g/kg microcystin (MC) extracts. Ultrastructural observation showed widened intercellular junction, distention of mitochondria, endoplasmic reticulum, and Golgi apparatus. All these changes appeared at 1, 3, and 12 h, but recovered finally. In biochemical analyses, the levels of lipid peroxidation (MDA) and H2O2 increased significantly at 1 h, indicating MC-caused oxidative stress. Finally, H2O2 decreased to the normal levels, while MDA remained at high levels. The antioxidative enzymes (CAT, SOD, GPx, GST) and antioxidants (GSH) also increased rapidly at 1 h, demonstrating a quick response of the defense systems to the oxidative stress. Finally, the activity of CAT, SOD, and GPX recovered to the normal level, while the activity of GST and the concentration of GSH remained at a high level. This suggests that the importance of MCs detoxification by GST via GSH, and the testis of rabbit contained abundant GSH. The final recovery of ultrastructure and some biochemical indexes indicates that the defense systems finally succeeded in protecting the testis against oxidative damage. In conclusion, these results indicate that the MCs are toxic to the male rabbit reproductive system and the mechanism underlying this toxicity might to be the oxidative stress caused by MCs. Although the negative effects of MCs can be overcome by the antioxidant system of testis in this study, the potential reproductive risks of l should not be neglected because of their wide occurrence. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 9-17. 2010. |
| 英文摘要 | In the present research, the changes of ultrastructures and biochemical index in rabbit testis were examined after i.p. injection with 12.5 mu g/kg microcystin (MC) extracts. Ultrastructural observation showed widened intercellular junction, distention of mitochondria, endoplasmic reticulum, and Golgi apparatus. All these changes appeared at 1, 3, and 12 h, but recovered finally. In biochemical analyses, the levels of lipid peroxidation (MDA) and H(2)O(2) increased significantly at 1 h, indicating MC-caused oxidative stress. Finally, H(2)O(2) decreased to the normal levels, while MDA remained at high levels. The antioxidative enzymes (CAT, SOD, GPx, GST) and antioxidants (GSH) also increased rapidly at 1 h, demonstrating a quick response of the defense systems to the oxidative stress. Finally, the activity of CAT, SOD, and GPX recovered to the normal level, while the activity of GST and the concentration of GSH remained at a high level. This suggests that the importance of MCs detoxification by GST via GSH, and the testis of rabbit contained abundant GSH. The final recovery of ultrastructure and some biochemical indexes indicates that the defense systems finally succeeded in protecting the testis against oxidative damage. In conclusion, these results indicate that the MCs are toxic to the male rabbit reproductive system and the mechanism underlying this toxicity might to be the oxidative stress caused by MCs. Although the negative effects of MCs can be overcome by the antioxidant system of testis in this study, the potential reproductive risks of l should not be neglected because of their wide occurrence. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 9-17. 2010. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Environmental Sciences; Toxicology; Water Resources |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | GLUTATHIONE S-TRANSFERASES ; OXIDATIVE STRESS ; IN-VIVO ; CYANOBACTERIA EXTRACT ; HEPATOCYTES ; LIVER ; DISRUPTION ; TRANSPORT ; SYSTEM ; TOXINS |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30530170] |
| 语种 | 英语 |
| WOS记录号 | WOS:000274715200002 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13554]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Xie, Ping,Qiu, Tong,et al. Microcystin Extracts Induce Ultrastructural Damage and Biochemical Disturbance in Male Rabbit Testis[J]. ENVIRONMENTAL TOXICOLOGY,2010,25(1):9-17. |
| APA | Liu, Ying.,Xie, Ping.,Qiu, Tong.,Li, Hui-Ying.,Li, Guang-Yu.,...&Xiong, Qian.(2010).Microcystin Extracts Induce Ultrastructural Damage and Biochemical Disturbance in Male Rabbit Testis.ENVIRONMENTAL TOXICOLOGY,25(1),9-17. |
| MLA | Liu, Ying,et al."Microcystin Extracts Induce Ultrastructural Damage and Biochemical Disturbance in Male Rabbit Testis".ENVIRONMENTAL TOXICOLOGY 25.1(2010):9-17. |
入库方式： OAI收割
来源：水生生物研究所
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