Acute Effects of Microcystins Exposure on the Transcription of Antioxidant Enzyme Genes in Three Organs (Liver, Kidney, and Testis) of Male Wistar Rats
文献类型：期刊论文
| 作者 | Xiong, Qian1,2; Xie, Ping1; Li, Huiying1; Hao, Le1,2; Li, Guangyu1,2; Qiu, Tong1,2; Liu, Ying1,2 |
| 刊名 | JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 24期号:6页码:361-367 |
| 关键词 | Microcystin Antioxidant Enzyme Wistar Rat Quantitative Real-Time PCR Oxidative Stress |
| ISSN号 | 1095-6670 |
| 通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China ; xieping@ihb.ac.cn |
| 中文摘要 | Microcystins (MCs) induce the production of reactive oxygen species (ROS) in various tissues in mammals, whereas the endogenous antioxidant enzymes are responsible to scavenge the ROS. ROS can modulate the antioxidant enzyme activities by regulating the mRNA levels. The present study was undertaken to find out the relationship between the transcriptional alterations of antioxidant enzymes and MCs stimulation in rats. The time-dependent changes of relative transcription abundance of catalase (CAT), Mn-superoxide dismutase (Mn-SOD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), glutathione reductase (GR), glutathione peroxidase (GPx), and gamma-glutamylcysteine synthetase (gamma-GCS) were investigated in three organs (liver, kidney, and testis) of male Wistar rats injected intravenously (i.v.) with 80 mu g MC-LRequivalent/kg body weight using the quantitative real-time PCR (qPCR) method. We found that MCs could affect the transcriptional activities of these antioxidant enzymes in liver, kidney, and testis of MCs-treated rats and we speculated the possible causation of the transcriptional change. The altered transcription of antioxidant enzymes may play an important role in counteracting the potential deleterious effects of elevated oxidative stress induced by MCs, and this will provide us new insights into the possible role of antioxidant enzymes in the toxicological mechanisms of MCs at molecular level. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:361-367, 2010; View this article online at wileyonlinelibrary.com. DOI 10;1002/jbt.20347 |
| 英文摘要 | Microcystins (MCs) induce the production of reactive oxygen species (ROS) in various tissues in mammals, whereas the endogenous antioxidant enzymes are responsible to scavenge the ROS. ROS can modulate the antioxidant enzyme activities by regulating the mRNA levels. The present study was undertaken to find out the relationship between the transcriptional alterations of antioxidant enzymes and MCs stimulation in rats. The time-dependent changes of relative transcription abundance of catalase (CAT), Mn-superoxide dismutase (Mn-SOD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), glutathione reductase (GR), glutathione peroxidase (GPx), and gamma-glutamylcysteine synthetase (gamma-GCS) were investigated in three organs (liver, kidney, and testis) of male Wistar rats injected intravenously (i.v.) with 80 mu g MC-LR(equivalent)/kg body weight using the quantitative real-time PCR (qPCR) method. We found that MCs could affect the transcriptional activities of these antioxidant enzymes in liver, kidney, and testis of MCs-treated rats and we speculated the possible causation of the transcriptional change. The altered transcription of antioxidant enzymes may play an important role in counteracting the potential deleterious effects of elevated oxidative stress induced by MCs, and this will provide us new insights into the possible role of antioxidant enzymes in the toxicological mechanisms of MCs at molecular level. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:361-367, 2010; View this article online at wileyonlinelibrary.com. DOI 10;1002/jbt.20347 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Toxicology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Toxicology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Toxicology |
| 关键词[WOS] | HYDROPEROXIDE GLUTATHIONE-PEROXIDASE ; INDUCED DNA-DAMAGE ; OXIDATIVE STRESS ; IN-VIVO ; REPRODUCTIVE-SYSTEM ; SKELETAL-MUSCLE ; PEPTIDE TOXIN ; LR ; EXPRESSION ; CYANOBACTERIA |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [30623001] |
| 语种 | 英语 |
| WOS记录号 | WOS:000286154800003 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15480]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Qian,Xie, Ping,Li, Huiying,et al. Acute Effects of Microcystins Exposure on the Transcription of Antioxidant Enzyme Genes in Three Organs (Liver, Kidney, and Testis) of Male Wistar Rats[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2010,24(6):361-367. |
| APA | Xiong, Qian.,Xie, Ping.,Li, Huiying.,Hao, Le.,Li, Guangyu.,...&Liu, Ying.(2010).Acute Effects of Microcystins Exposure on the Transcription of Antioxidant Enzyme Genes in Three Organs (Liver, Kidney, and Testis) of Male Wistar Rats.JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,24(6),361-367. |
| MLA | Xiong, Qian,et al."Acute Effects of Microcystins Exposure on the Transcription of Antioxidant Enzyme Genes in Three Organs (Liver, Kidney, and Testis) of Male Wistar Rats".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 24.6(2010):361-367. |
入库方式： OAI收割
来源：水生生物研究所
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