Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR
文献类型：期刊论文
| 作者 | Li, Guangyu1; Chen, Jun1; Xie, Ping1; Jiang, Yan1; Wu, Laiyan1; Zhang, Xuezhen2 |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 11期号:10页码:2003-2018 |
| 关键词 | Animal proteomics Developmental toxicity Gene expression Microcystin-leucine-arginine Zebrafish |
| ISSN号 | 1615-9853 |
| 通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China ; xieping@ihb.ac.cn |
| 中文摘要 | Microcystin-leucine-arginine (MCLR) is the most toxic and the most commonly encountered variant of microcystins (MCs) in aquatic environment, and it has the potential for developmental toxicity. A number of previous studies have described the developing toxicity of MCLR based on conventional toxicological indices. However, the molecular mechanisms by which it expresses its toxicity during the early development remain largely unknown. To further our understanding of mechanisms of action and identify the potential protein biomarkers for MCLR exposure, a proteomic analysis was performed on developing zebrafish embryos exposed to 0.5 mg/L MCLR until 96 hours post-fertilization. 2-DE combined with MS was employed to detect and identify the protein profiles. Results showed that 75 spots from the 0.5 mg/L MCLR condition showed a significant increase or decrease in abundance compared with the control. In total, 40 proteins were identified. These proteins were mainly included in process related to oxidative stress, energetic metabolism, and the cytoskeleton assembly. MCLR exposure also affects the expression of the subunits of protein phosphatases 2A. Furthermore, the proteomic and transcriptional analysis of nine proteins was determined by Western blot and quantitative real-time PCR due to their correlation with the known MCLR toxic mechanisms. The consistent and discrepant results between protein and mRNA levels indicated complicated regulatory mechanisms of gene expression in response to MCLR exposure. |
| 英文摘要 | Microcystin-leucine-arginine (MCLR) is the most toxic and the most commonly encountered variant of microcystins (MCs) in aquatic environment, and it has the potential for developmental toxicity. A number of previous studies have described the developing toxicity of MCLR based on conventional toxicological indices. However, the molecular mechanisms by which it expresses its toxicity during the early development remain largely unknown. To further our understanding of mechanisms of action and identify the potential protein biomarkers for MCLR exposure, a proteomic analysis was performed on developing zebrafish embryos exposed to 0.5 mg/L MCLR until 96 hours post-fertilization. 2-DE combined with MS was employed to detect and identify the protein profiles. Results showed that 75 spots from the 0.5 mg/L MCLR condition showed a significant increase or decrease in abundance compared with the control. In total, 40 proteins were identified. These proteins were mainly included in process related to oxidative stress, energetic metabolism, and the cytoskeleton assembly. MCLR exposure also affects the expression of the subunits of protein phosphatases 2A. Furthermore, the proteomic and transcriptional analysis of nine proteins was determined by Western blot and quantitative real-time PCR due to their correlation with the known MCLR toxic mechanisms. The consistent and discrepant results between protein and mRNA levels indicated complicated regulatory mechanisms of gene expression in response to MCLR exposure. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemical Research Methods; Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | KERATIN INTERMEDIATE-FILAMENTS ; PROTEOMIC ANALYSIS ; OXIDATIVE STRESS ; ORYZIAS-LATIPES ; PHOSPHATASE 2A ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; LENS CRYSTALLINS ; CRUDE EXTRACTS ; FISH |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457] |
| 语种 | 英语 |
| WOS记录号 | WOS:000290486200012 |
| 公开日期 | 2011-06-29 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15578]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guangyu,Chen, Jun,Xie, Ping,et al. Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR[J]. PROTEOMICS,2011,11(10):2003-2018. |
| APA | Li, Guangyu,Chen, Jun,Xie, Ping,Jiang, Yan,Wu, Laiyan,&Zhang, Xuezhen.(2011).Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR.PROTEOMICS,11(10),2003-2018. |
| MLA | Li, Guangyu,et al."Protein expression profiling in the zebrafish (Danio rerio) embryos exposed to the microcystin-LR".PROTEOMICS 11.10(2011):2003-2018. |
入库方式： OAI收割
来源：水生生物研究所
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