Acute Effects of Microcystins on the Transcription of 14 Glutathione S-Transferase Isoforms in Wistar Rat
文献类型：期刊论文
| 作者 | Li, Guangyu; Xie, Ping; Li, Huiying; Hao, Le; Xiong, Qian; Qiu, Tong; Liu, Ying |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 26期号:2页码:187-194 |
| ISSN号 | 1520-4081 |
| 关键词 | microcystin glutathione S-transferases real time PCR gene expression Wistar rat |
| 通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China ; xieping@ihb.ac.cn |
| 中文摘要 | The glutathione S-transferases (GST) play important roles in the detoxification of microcystins (MCs). For better understanding of the responses of GST isforms to MCs exposure, informations about the effects of MCs on GSTs are necessary. In this experiment, we cloned the full length cDNA of 14 GST isoforms (GST alpha, kappa, mu, omega, pi, theta, zeta, and microsomal GST) from Wistar rat. The mRNA abundance of each rat GST isoform in the liver, kidney, and testis was analyzed by real time quantitative PCR. Multiple GST isoforms were constitutively expressed in all examined organs, but some isoforms were expressed at higher level in one organ than in others. The relative changes of the mRNA abundance in the liver, kidney, and testis of Wiatar rat i.v. injected with crude MCs extract at dose of 1LD(50) were also analyzed. Generally, the expression of most GSTs in the liver and testis was suppressed while that in kidney was induced after being injected with MCs. It is suggested that the transcription of GST isoforms varied in different ways within an organ and between organs of Wistar rat exposed to MCs. (c) 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 187-194, 2011. |
| 英文摘要 | The glutathione S-transferases (GST) play important roles in the detoxification of microcystins (MCs). For better understanding of the responses of GST isforms to MCs exposure, informations about the effects of MCs on GSTs are necessary. In this experiment, we cloned the full length cDNA of 14 GST isoforms (GST alpha, kappa, mu, omega, pi, theta, zeta, and microsomal GST) from Wistar rat. The mRNA abundance of each rat GST isoform in the liver, kidney, and testis was analyzed by real time quantitative PCR. Multiple GST isoforms were constitutively expressed in all examined organs, but some isoforms were expressed at higher level in one organ than in others. The relative changes of the mRNA abundance in the liver, kidney, and testis of Wiatar rat i.v. injected with crude MCs extract at dose of 1LD(50) were also analyzed. Generally, the expression of most GSTs in the liver and testis was suppressed while that in kidney was induced after being injected with MCs. It is suggested that the transcription of GST isoforms varied in different ways within an organ and between organs of Wistar rat exposed to MCs. (c) 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 187-194, 2011. |
| 学科主题 | Environmental Sciences; Toxicology; Water Resources |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | GOLDFISH CARASSIUS-AURATUS ; CARP CYPRINUS-CARPIO ; BUTYLATED HYDROXYANISOLE ; ANTIOXIDANT RESPONSE ; PROTEIN PHOSPHATASE ; TISSUE DISTRIBUTION ; CORYDORAS-PALEATUS ; GENE-EXPRESSION ; DANIO-RERIO ; REAL-TIME |
| 资助信息 | National Natural Science Foundation of China [30700077, 30530170] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288652000009 |
| 公开日期 | 2011-06-30 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15606]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guangyu,Xie, Ping,Li, Huiying,et al. Acute Effects of Microcystins on the Transcription of 14 Glutathione S-Transferase Isoforms in Wistar Rat[J]. ENVIRONMENTAL TOXICOLOGY,2011,26(2):187-194. |
| APA | Li, Guangyu.,Xie, Ping.,Li, Huiying.,Hao, Le.,Xiong, Qian.,...&Liu, Ying.(2011).Acute Effects of Microcystins on the Transcription of 14 Glutathione S-Transferase Isoforms in Wistar Rat.ENVIRONMENTAL TOXICOLOGY,26(2),187-194. |
| MLA | Li, Guangyu,et al."Acute Effects of Microcystins on the Transcription of 14 Glutathione S-Transferase Isoforms in Wistar Rat".ENVIRONMENTAL TOXICOLOGY 26.2(2011):187-194. |
入库方式： OAI收割
来源：水生生物研究所
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