Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses
文献类型：期刊论文
| 作者 | Shao, Jihai1,2,3; Yu, Gongliang1; Wang, Zhongjie1,3; Wu, Zhongxing1; Peng, Xin1; Li, Renhui1 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 19期号:8页码:1634-1641 |
| 关键词 | Wheat bran leachate Microcystis Gene expression Photosynthesis |
| ISSN号 | 0963-9292 |
| 通讯作者 | Li, RH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Wheat bran leachate (WBL) has been shown to have an inhibitory effect on Microcystis aeruginosa in this study. In order to explore the inhibitory mechanism of WBL on M. aeruginosa, physiological responses of M. aeruginosa NIES-843 under the WBL stress were studied. The expressions of six important genes related to the D1 protein of photosynthetic processes (psbA), synthesis of microcystins (mcyB), antioxidant protein peroxiredoxin (prx), synthesis of fatty acid (fabZ) and the repair of biological macromolecules (recA, grpE) were studied. Under the WBL stress, no significant change was found in expressions of both grpE and recA, but expressions of psbA, fabZ and prx were shown to be down-regulated, and slight up-regulated expression was found in mcyB. It was shown that oxygen evolution of M. aeruginosa NIES-843 was significantly depressed, and intracellular ATP contents became lower, after exposure to WBL. Similarly, maximum electron transport rates of photosynthetic activities decreased significantly, but intracellular reactive oxygen species levels boosted dramatically under the WBL stress, and cell lysis was observed. Therefore, it is suggested that photosynthetic systems and membranes were the potential targets of toxicity of WBL on M. aeruginosa, and the oxidative damage is an important mechanism explaining the inhibitory effect of WBL on M. aeruginosa. |
| 英文摘要 | Wheat bran leachate (WBL) has been shown to have an inhibitory effect on Microcystis aeruginosa in this study. In order to explore the inhibitory mechanism of WBL on M. aeruginosa, physiological responses of M. aeruginosa NIES-843 under the WBL stress were studied. The expressions of six important genes related to the D1 protein of photosynthetic processes (psbA), synthesis of microcystins (mcyB), antioxidant protein peroxiredoxin (prx), synthesis of fatty acid (fabZ) and the repair of biological macromolecules (recA, grpE) were studied. Under the WBL stress, no significant change was found in expressions of both grpE and recA, but expressions of psbA, fabZ and prx were shown to be down-regulated, and slight up-regulated expression was found in mcyB. It was shown that oxygen evolution of M. aeruginosa NIES-843 was significantly depressed, and intracellular ATP contents became lower, after exposure to WBL. Similarly, maximum electron transport rates of photosynthetic activities decreased significantly, but intracellular reactive oxygen species levels boosted dramatically under the WBL stress, and cell lysis was observed. Therefore, it is suggested that photosynthetic systems and membranes were the potential targets of toxicity of WBL on M. aeruginosa, and the oxidative damage is an important mechanism explaining the inhibitory effect of WBL on M. aeruginosa. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Ecology; Environmental Sciences; Toxicology |
| 类目[WOS] | Ecology ; Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | BARLEY-STRAW ; OXIDATIVE STRESS ; ANTIALGAL ACTIVITY ; GROWTH-INHIBITION ; ESCHERICHIA-COLI ; GENE-EXPRESSION ; PHOTOSYSTEM-II ; ALGAL GROWTH ; PEROXIREDOXINS ; QUANTIFICATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (NSFC) [30821140542]; National Water Science and Technology Projects [2009ZX07101-013-02, 2008ZX07105-006]; Chinese Academy of Sciences [082303-1-501] |
| 语种 | 英语 |
| WOS记录号 | WOS:000284363800027 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13757]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, Jihai,Yu, Gongliang,Wang, Zhongjie,et al. Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses[J]. ECOTOXICOLOGY,2010,19(8):1634-1641. |
| APA | Shao, Jihai,Yu, Gongliang,Wang, Zhongjie,Wu, Zhongxing,Peng, Xin,&Li, Renhui.(2010).Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses.ECOTOXICOLOGY,19(8),1634-1641. |
| MLA | Shao, Jihai,et al."Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses".ECOTOXICOLOGY 19.8(2010):1634-1641. |
入库方式： OAI收割
来源：水生生物研究所
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