cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)
文献类型：期刊论文
| 作者 | Tan, Wenhua1,2; Liu, Yang1,2; Wu, Zhongxing1; Lin, Shen1,2; Yu, Gongliang1; Yu, Boshi1; Li, Renhui1 |
| 刊名 | HARMFUL ALGAE
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 9期号:6页码:607-612 |
| 关键词 | cpcBA-IGS Cyanobacteria ITS Microcystis wesenbergii Phylogeny Water bloom |
| ISSN号 | 1568-9883 |
| 通讯作者 | Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved. |
| 英文摘要 | Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Marine & Freshwater Biology |
| 类目[WOS] | Marine & Freshwater Biology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | RIBOSOMAL-RNA SEQUENCES ; GENUS MICROCYSTIS ; PHYLOGENETIC-RELATIONSHIPS ; GENETIC DIVERSITY ; LAKE WANNSEE ; WATER BODIES ; STRAINS ; CYANOPHYTA ; AERUGINOSA ; 16S |
| 收录类别 | SCI |
| 资助信息 | State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] |
| 语种 | 英语 |
| WOS记录号 | WOS:000281999300010 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13771]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Wenhua,Liu, Yang,Wu, Zhongxing,et al. cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)[J]. HARMFUL ALGAE,2010,9(6):607-612. |
| APA | Tan, Wenhua.,Liu, Yang.,Wu, Zhongxing.,Lin, Shen.,Yu, Gongliang.,...&Li, Renhui.(2010).cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria).HARMFUL ALGAE,9(6),607-612. |
| MLA | Tan, Wenhua,et al."cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)".HARMFUL ALGAE 9.6(2010):607-612. |
入库方式： OAI收割
来源：水生生物研究所
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