CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120
文献类型：期刊论文
| 作者 | He, Dongli; Xu, Xudong |
| 刊名 | ARCHIVES OF MICROBIOLOGY
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 192期号:6页码:461-469 |
| 关键词 | CalA ftsZ DNA-binding protein Anabaena |
| ISSN号 | 0302-8933 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | A protein of cyAbrB family of regulators, Alr0946 (CalA), was identified by DNA affinity chromatography using DNA fragments from the promoter region of ftsZ in Anabaena/Nostoc sp. PCC 7120. Electrophoretic mobility shift assays showed that the protein could bind to upstream regions of ftsZ (as biotin-labeled DNA fragments) and that the binding was inhibited by the same DNA fragments and a fragment upstream of hetC but not by 3 fragments upstream of glnA, hetR or ntcA. Both transcriptional fusion with gfp (green fluorescence protein) and Western blot analysis indicated that the expression of calA was down-regulated in heterocysts relative to vegetative cells. An insertional mutant of calA could not be completely segregated. Over-expression of calA in Anabaena/Nostoc caused no change in growth, heterocyst differentiation or expression of ftsZ. CalA as a DNA-binding protein is essential for the growth of Anabaena/Nostoc and may be required for the expression of ftsZ in vegetative cells. |
| 英文摘要 | A protein of cyAbrB family of regulators, Alr0946 (CalA), was identified by DNA affinity chromatography using DNA fragments from the promoter region of ftsZ in Anabaena/Nostoc sp. PCC 7120. Electrophoretic mobility shift assays showed that the protein could bind to upstream regions of ftsZ (as biotin-labeled DNA fragments) and that the binding was inhibited by the same DNA fragments and a fragment upstream of hetC but not by 3 fragments upstream of glnA, hetR or ntcA. Both transcriptional fusion with gfp (green fluorescence protein) and Western blot analysis indicated that the expression of calA was down-regulated in heterocysts relative to vegetative cells. An insertional mutant of calA could not be completely segregated. Over-expression of calA in Anabaena/Nostoc caused no change in growth, heterocyst differentiation or expression of ftsZ. CalA as a DNA-binding protein is essential for the growth of Anabaena/Nostoc and may be required for the expression of ftsZ in vegetative cells. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | SP-STRAIN PCC-7120 ; CELL-DIVISION ; BACILLUS-SUBTILIS ; GENE-EXPRESSION ; TRANSCRIPTIONAL REGULATOR ; DIFFERENTIATION ; HETR ; NTCA ; CYANOBACTERIA ; TRANSITION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30570022, 30770049] |
| 语种 | 英语 |
| WOS记录号 | WOS:000277785300005 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13789]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Dongli,Xu, Xudong. CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120[J]. ARCHIVES OF MICROBIOLOGY,2010,192(6):461-469. |
| APA | He, Dongli,&Xu, Xudong.(2010).CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120.ARCHIVES OF MICROBIOLOGY,192(6),461-469. |
| MLA | He, Dongli,et al."CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120".ARCHIVES OF MICROBIOLOGY 192.6(2010):461-469. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。