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Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China

文献类型：期刊论文

作者Lin, Shen1,4; Shen, Jianzhong2; Liu, Yang1,4; Wu, Xingqiang1,4; Liu, Qigen3; Li, Renhui1
刊名ENVIRONMENTAL MONITORING AND ASSESSMENT
出版日期2011-04-01
卷号175期号:1-4页码:139-150
关键词Lake Ulungur Microcystis Real-time PCR HPLC Microcystin Mcy gene Brackish lake
ISSN号0167-6369
通讯作者Li, R.(reli@ihb.ac.cn)
中文摘要The distribution and diversity of the waterbloom-forming cyanobacteria, mainly including Microcystis species, were investigated using molecular approaches in Lake Ulungur, an increasingly eutrophic and brackish lake located in Xinjiang, China. Real-time PCR analyses showed the abundance of Microcystis 16S rDNA gene copies in the Ulungur Lake is low, at 7.2 × 104 copies L - 1 averagely and 4.5 × 105 copies L - 1 at maximum. Two Microcystis species, M. aeruginosa (Ku¨tzing) Lemmermann and Microcystis wesenbergii (Koma´rek) Koma´rek were, for the first time, reported in this lake. The mcyA gene-specific PCR determination on the isolates of Microcystis showed that the M. aeruginosa strains are all mcyA-containing genotypes, while M. wesenbergii are non-mcyA-containing ones. The microcystin contents of the toxic M. aeruginosa strains were shown to be lower than those of the Microcystis strains isolated from other eutrophic lakes in China. Phylogenetic analyses based on 16S rRNA and rpoC1 genes showed that the Microcystis strains isolated from the Ulungur Lake were not genetically divergent from those isolated in the other freshwaters. Such an investigation would contribute to the knowledge on the bloom-forming cyanobacteria of the increasingly eutrophic and saline lakes in the desert area. © 2010 Springer Science+Business Media B.V.
英文摘要The distribution and diversity of the waterbloom-forming cyanobacteria, mainly including Microcystis species, were investigated using molecular approaches in Lake Ulungur, an increasingly eutrophic and brackish lake located in Xinjiang, China. Real-time PCR analyses showed the abundance of Microcystis 16S rDNA gene copies in the Ulungur Lake is low, at 7.2 x 10(4) copies L (-aEuro parts per thousand 1) averagely and 4.5 x 10(5) copies L (-aEuro parts per thousand 1) at maximum. Two Microcystis species, M. aeruginosa (Kutzing) Lemmermann and Microcystis wesenbergii (Komarek) Komarek were, for the first time, reported in this lake. The mcyA gene-specific PCR determination on the isolates of Microcystis showed that the M. aeruginosa strains are all mcyA-containing genotypes, while M. wesenbergii are non-mcyA-containing ones. The microcystin contents of the toxic M. aeruginosa strains were shown to be lower than those of the Microcystis strains isolated from other eutrophic lakes in China. Phylogenetic analyses based on 16S rRNA and rpoC1 genes showed that the Microcystis strains isolated from the Ulungur Lake were not genetically divergent from those isolated in the other freshwaters. Such an investigation would contribute to the knowledge on the bloom-forming cyanobacteria of the increasingly eutrophic and saline lakes in the desert area.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Environmental Sciences
研究领域[WOS]Environmental Sciences & Ecology
关键词[WOS]REAL-TIME PCR ; GENUS MICROCYSTIS ; BLOOMS ; BIOSYNTHESIS ; EVOLUTION ; SEQUENCES ; GENOTYPES ; WATER
收录类别SCI
语种英语
WOS记录号WOS:000288026600012
公开日期2011-03-14
源URL[http://ir.ihb.ac.cn/handle/342005/14444]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 200090, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Lin, Shen,Shen, Jianzhong,Liu, Yang,et al. Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China[J]. ENVIRONMENTAL MONITORING AND ASSESSMENT,2011,175(1-4):139-150.
APA Lin, Shen,Shen, Jianzhong,Liu, Yang,Wu, Xingqiang,Liu, Qigen,&Li, Renhui.(2011).Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China.ENVIRONMENTAL MONITORING AND ASSESSMENT,175(1-4),139-150.
MLA Lin, Shen,et al."Molecular evaluation on the distribution, diversity, and toxicity of Microcystis (Cyanobacteria) species from Lake Ulungur-a mesotrophic brackish desert lake in Xinjiang, China".ENVIRONMENTAL MONITORING AND ASSESSMENT 175.1-4(2011):139-150.

入库方式： OAI收割

来源：水生生物研究所

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