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Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae

文献类型:期刊论文

作者Chen, Wei1,2; Sommerfeld, Milton1; Hu, Qiang1
刊名BIORESOURCE TECHNOLOGY
出版日期2011
卷号102期号:1页码:135-141
ISSN号0960-8524
关键词Biofuel Microwave-assisted staining Green algae Neutral lipids Nile red
通讯作者Hu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA ; huqiang@asu.edu
中文摘要In vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd.
英文摘要In vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd.
学科主题Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]RAPID SCREENING METHOD ; SPECTROFLUOROMETRIC QUANTIFICATION ; POLAR LIPIDS ; IRRADIATION ; BIOTECHNOLOGY ; FLUORESCENCE ; SPECIMENS ; GROWTH ; ALGAE ; CELLS
收录类别SCI
语种英语
WOS记录号WOS:000285658300020
公开日期2011-04-14
源URL[http://ir.ihb.ac.cn/handle/342005/15542]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位1.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Chen, Wei,Sommerfeld, Milton,Hu, Qiang. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae[J]. BIORESOURCE TECHNOLOGY,2011,102(1):135-141.
APA Chen, Wei,Sommerfeld, Milton,&Hu, Qiang.(2011).Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae.BIORESOURCE TECHNOLOGY,102(1),135-141.
MLA Chen, Wei,et al."Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae".BIORESOURCE TECHNOLOGY 102.1(2011):135-141.

入库方式: OAI收割

来源:水生生物研究所

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