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Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae
文献类型:期刊论文
作者 | Chen, Wei1,2; Sommerfeld, Milton1; Hu, Qiang1 |
刊名 | BIORESOURCE TECHNOLOGY |
出版日期 | 2011 |
卷号 | 102期号:1页码:135-141 |
ISSN号 | 0960-8524 |
关键词 | Biofuel Microwave-assisted staining Green algae Neutral lipids Nile red |
通讯作者 | Hu, QA, Arizona State Univ, Dept Appl Sci & Math, 7001 E Williams Field Rd, Mesa, AZ 85212 USA ; huqiang@asu.edu |
中文摘要 | In vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd. |
英文摘要 | In vivo determination of neutral lipids with Nile red fluorescence has been used as a rapid screening method for certain types of microalgae, but has been unsuccessful in others, particularly those with thick, rigid cell walls that prevent penetration of the fluorescence dye into the cell. To solve the problem, a microwave-assisted Nile red staining method for microalgal lipid determination was developed. In a two-step staining protocol, 50 and 60 s were selected as the optimal microwave times for the pretreatment and staining process, respectively. Moreover, several calibration methods for quantitative analysis of neutral lipids in microalgae were investigated and compared with conventional gravimetric methods. Factors that affected the in vivo quantification of cellular neutral lipids were also investigated. Application of the new method for detection and quantification of neutral lipids in a number of green microalgae was demonstrated. Published by Elsevier Ltd. |
学科主题 | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
关键词[WOS] | RAPID SCREENING METHOD ; SPECTROFLUOROMETRIC QUANTIFICATION ; POLAR LIPIDS ; IRRADIATION ; BIOTECHNOLOGY ; FLUORESCENCE ; SPECIMENS ; GROWTH ; ALGAE ; CELLS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000285658300020 |
公开日期 | 2011-04-14 |
源URL | [http://ir.ihb.ac.cn/handle/342005/15542] |
专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
作者单位 | 1.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Wei,Sommerfeld, Milton,Hu, Qiang. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae[J]. BIORESOURCE TECHNOLOGY,2011,102(1):135-141. |
APA | Chen, Wei,Sommerfeld, Milton,&Hu, Qiang.(2011).Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae.BIORESOURCE TECHNOLOGY,102(1),135-141. |
MLA | Chen, Wei,et al."Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae".BIORESOURCE TECHNOLOGY 102.1(2011):135-141. |
入库方式: OAI收割
来源:水生生物研究所
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