Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts
文献类型：期刊论文
| 作者 | Lan, Shubin1,2; Wu, Li1,2; Zhang, Delu1,3; Hu, Chunxiang1; Liu, Yongding1 |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 43期号:4页码:857-861 |
| 关键词 | Photoautotrophic biomass Extraction efficiency Mass of sample Development and succession Soil characteristics |
| ISSN号 | 0038-0717 |
| 通讯作者 | Hu, CX, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; cxhu@ihb.ac.cn |
| 中文摘要 | In order to estimate the biomass of photoautotrophic organisms in biological soil crusts (BSCs), based on the extraction and determination of phytoplanktonic chlorophyll-a (Chl-a) in aquatic ecosystems, this paper comparatively studied the Chl-a extraction efficiencies in ethanol, acetone, N. N -dimethyl -formamide (DMF) and dimethyl sulphoxide (DMSO) from algae, lichen, and moss crusts, analyzed the effects of dominant organisms or development degree of BSCs, mass of sample, and soil characteristics on the extraction efficiency. The results showed that the extraction efficiencies of different organic solvents were significantly different, and such efficiencies declined with the increase of mass of sample. DMSO resulted in the greatest extraction efficiency, but was not suitable for lichen crusts; DMF strongly underestimated the Chl-a content of man-made crusts relative to the other extractants, and was particularly affected by interfering pigments from the well-developed crusts. Generally, the extraction efficiency of ethanol was greater than that of acetone, and ethanol method showed the greatest stability compared with other 3 solvents. In addition, Chia content showed an increasing trend with the succession of BSCs when it was expressed on an areal basis. On the basis of this and related experiments we suggest Chl-a content of BSCs be extracted by ethanol and expressed on an areal basis. (C) 2010 Elsevier Ltd. All rights reserved. |
| 英文摘要 | In order to estimate the biomass of photoautotrophic organisms in biological soil crusts (BSCs), based on the extraction and determination of phytoplanktonic chlorophyll-a (Chl-a) in aquatic ecosystems, this paper comparatively studied the Chl-a extraction efficiencies in ethanol, acetone, N. N -dimethyl -formamide (DMF) and dimethyl sulphoxide (DMSO) from algae, lichen, and moss crusts, analyzed the effects of dominant organisms or development degree of BSCs, mass of sample, and soil characteristics on the extraction efficiency. The results showed that the extraction efficiencies of different organic solvents were significantly different, and such efficiencies declined with the increase of mass of sample. DMSO resulted in the greatest extraction efficiency, but was not suitable for lichen crusts; DMF strongly underestimated the Chl-a content of man-made crusts relative to the other extractants, and was particularly affected by interfering pigments from the well-developed crusts. Generally, the extraction efficiency of ethanol was greater than that of acetone, and ethanol method showed the greatest stability compared with other 3 solvents. In addition, Chia content showed an increasing trend with the succession of BSCs when it was expressed on an areal basis. On the basis of this and related experiments we suggest Chl-a content of BSCs be extracted by ethanol and expressed on an areal basis. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Soil Science |
| 类目[WOS] | Soil Science |
| 研究领域[WOS] | Agriculture |
| 关键词[WOS] | ALGAL CRUSTS ; INNER-MONGOLIA ; MICROBIOTIC CRUSTS ; DESERT CRUSTS ; CYANOBACTERIAL ; ECOSYSTEM ; BIOMASS ; CHINA ; UTAH |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30870470]; National Principal Expert Program [2008ZX07103-004, 2009ZX07106-001-003, 2009ZX07210-009, 2008ZX07103-007]; Wuhan Science and Technology Bureau ; Inner Mongolia Planning Committee on high-tech industrialization |
| 语种 | 英语 |
| WOS记录号 | WOS:000288571700019 |
| 公开日期 | 2011-06-30 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15686]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wuhan Univ Technol, Dept Biol Sci & Biotechnol, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lan, Shubin,Wu, Li,Zhang, Delu,et al. Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts[J]. SOIL BIOLOGY & BIOCHEMISTRY,2011,43(4):857-861. |
| APA | Lan, Shubin,Wu, Li,Zhang, Delu,Hu, Chunxiang,&Liu, Yongding.(2011).Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts.SOIL BIOLOGY & BIOCHEMISTRY,43(4),857-861. |
| MLA | Lan, Shubin,et al."Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts".SOIL BIOLOGY & BIOCHEMISTRY 43.4(2011):857-861. |
入库方式： OAI收割
来源：水生生物研究所
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