Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella
文献类型：期刊论文
| 作者 | Xiao, F. S.1,2; Wang, Y. P.1; Yan, W.1; Chang, M. X.1; Yao, W. J.1; Xu, Q. Q.1; Wang, X. X.1; Gao, Q.1; Nie, P.1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 29期号:4页码:594-599 |
| 关键词 | Immunoglobulin (Ig) IgZ IgM IgD IgH locus Grass carp Ctenopharyngodon idella |
| ISSN号 | 1050-4648 |
| 通讯作者 | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | The cDNA and genomic sequences of IgD and IgZ were characterized in grass carp Ctenopharyngodon idella in the present study, and with the identification of a BAC clone covering zeta, mu, and delta genes, the IgH locus containing these Ig genes and other V, D, J genes was also illustrated in this fish. Secretory and membrane-bound IgZ were identified, with two transmembrane exons spliced within the CH4 exon, as reported in IgM of mammals and IgZ in other teleost fish. The first and second constant domains of IgZ shows more than 90% nucleotide identity with respective domains of grass carp IgM. The IgD has a structure of delta 1-(delta 2-delta 3-delta 4)(2)-delta 5-delta 6-delta 7-TM-UTR, with the repeat of delta 2-delta 3-delta 4; but intron was not found between the two repeat, i.e. between the first delta 2-delta 3-delta 4 (delta 2.1-delta 3.1-delta 4.1) and the second delta 2-delta 3-delta 4 (delta 2.2-delta 3.2-delta 4.2), and the intron between delta 3.1 and delta 4.1 was much shorter than the intron between delta 3.2 and delta 4.2. The genomic organization of the IgH locus has a pattern of Vn-Dn-Jn-C zeta-Dn-Jn-C mu-C delta, as reported in other teleost fish. Thirteen V-H, fourteen D, and twelve J(H) genes were observed in this locus, with the similarity of three D segments and four J(H) segments being the same in the upstream of C zeta, and C mu. The transcriptional enhancer located at the mu-delta intergenic region was also analyzed and it seems possible that this enhancer is functional as verified in zebrafish and channel catfish. (C) 2010 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The cDNA and genomic sequences of IgD and IgZ were characterized in grass carp Ctenopharyngodon idella in the present study, and with the identification of a BAC clone covering zeta, mu, and delta genes, the IgH locus containing these Ig genes and other V, D, J genes was also illustrated in this fish. Secretory and membrane-bound IgZ were identified, with two transmembrane exons spliced within the CH4 exon, as reported in IgM of mammals and IgZ in other teleost fish. The first and second constant domains of IgZ shows more than 90% nucleotide identity with respective domains of grass carp IgM. The IgD has a structure of delta 1-(delta 2-delta 3-delta 4)(2)-delta 5-delta 6-delta 7-TM-UTR, with the repeat of delta 2-delta 3-delta 4; but intron was not found between the two repeat, i.e. between the first delta 2-delta 3-delta 4 (delta 2.1-delta 3.1-delta 4.1) and the second delta 2-delta 3-delta 4 (delta 2.2-delta 3.2-delta 4.2), and the intron between delta 3.1 and delta 4.1 was much shorter than the intron between delta 3.2 and delta 4.2. The genomic organization of the IgH locus has a pattern of Vn-Dn-Jn-C zeta-Dn-Jn-C mu-C delta, as reported in other teleost fish. Thirteen V-H, fourteen D, and twelve J(H) genes were observed in this locus, with the similarity of three D segments and four J(H) segments being the same in the upstream of C zeta, and C mu. The transcriptional enhancer located at the mu-delta intergenic region was also analyzed and it seems possible that this enhancer is functional as verified in zebrafish and channel catfish. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | IMMUNOGLOBULIN GENES ; E-MU-3' ENHANCER ; CHANNEL CATFISH ; MESSENGER-RNA ; REGION GENES ; TELEOST FISH ; EXPRESSION ; ISOTYPE ; EVOLUTION ; DOMAINS |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Programme (973 Programme) of China [2009CB118703] |
| 语种 | 英语 |
| WOS记录号 | WOS:000281500500007 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13591]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, F. S.,Wang, Y. P.,Yan, W.,et al. Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(4):594-599. |
| APA | Xiao, F. S..,Wang, Y. P..,Yan, W..,Chang, M. X..,Yao, W. J..,...&Nie, P..(2010).Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella.FISH & SHELLFISH IMMUNOLOGY,29(4),594-599. |
| MLA | Xiao, F. S.,et al."Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella".FISH & SHELLFISH IMMUNOLOGY 29.4(2010):594-599. |
入库方式： OAI收割
来源：水生生物研究所
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