Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Luo, Yu-Shan1,2; Hu, Wei1; Liu, Xiao-Chun3; Lin, Hao-Ran3; Zhu, Zuo-Yan1 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2010-08-15 |
| 卷号 | 306期号:1-4页码:322-328 |
| 关键词 | Orange-spotted grouper Sox9 Sex reversal Expression pattern |
| ISSN号 | 0044-8486 |
| 通讯作者 | Hu, W, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Sox9 is a key gene in male sex determination and gonad development. To study its potential function in the female-to-male sex reversal in orange-spotted grouper, we conducted the following studies. The Sox9 gene was cloned and full-length sequence of Sox9 mRNA was determined using the rapid amplification of cDNA ends method. The Sox9 mRNA consists of 3277 bp in size with a 328 bp 5' untranslated region and a 1511 bp 3' untranslated region. The 1437 bp opening reading frame encodes a 479 amino acid protein. The Sox9 gene contains 3 exons and 2 introns; the beginning and end of both introns conform to the "GT-AG" rule. RT-PCR analysis indicated that Sox9 mRNA was expressed in brain, kidney, heart, liver, muscle, stomach, intestine, spleen, testis and ovary in adult orange-spotted grouper. Artificial sex reversal was successfully performed by implanting a medicinal strip containing 17 alpha-methyltestosterone into the groupers. By real-time PCR, we found that Sox9 was weakly expressed in the ovary-stage gonads (before treatment). Once the sex reversal began (1 week after treatment), Sox9 mRNA expression level significantly increased. However, at 2 weeks after treatment, Sox9 mRNA expression level significantly decreased to a level that was only slightly higher than that before treatment Sox9 mRNA expression increased again at 4 weeks after treatment, and at 6 and 8 weeks, it was maintained at a high level close to that at 1 week after treatment. The results suggest that Sox9 may be one of the important factors initiating and maintaining the masculinization of orange-spotted grouper during sex reversal. (C) 2010 Elsevier B.V. All rights reserved. |
| 英文摘要 | Sox9 is a key gene in male sex determination and gonad development. To study its potential function in the female-to-male sex reversal in orange-spotted grouper, we conducted the following studies. The Sox9 gene was cloned and full-length sequence of Sox9 mRNA was determined using the rapid amplification of cDNA ends method. The Sox9 mRNA consists of 3277 bp in size with a 328 bp 5' untranslated region and a 1511 bp 3' untranslated region. The 1437 bp opening reading frame encodes a 479 amino acid protein. The Sox9 gene contains 3 exons and 2 introns; the beginning and end of both introns conform to the "GT-AG" rule. RT-PCR analysis indicated that Sox9 mRNA was expressed in brain, kidney, heart, liver, muscle, stomach, intestine, spleen, testis and ovary in adult orange-spotted grouper. Artificial sex reversal was successfully performed by implanting a medicinal strip containing 17 alpha-methyltestosterone into the groupers. By real-time PCR, we found that Sox9 was weakly expressed in the ovary-stage gonads (before treatment). Once the sex reversal began (1 week after treatment), Sox9 mRNA expression level significantly increased. However, at 2 weeks after treatment, Sox9 mRNA expression level significantly decreased to a level that was only slightly higher than that before treatment Sox9 mRNA expression increased again at 4 weeks after treatment, and at 6 and 8 weeks, it was maintained at a high level close to that at 1 week after treatment. The results suggest that Sox9 may be one of the important factors initiating and maintaining the masculinization of orange-spotted grouper during sex reversal. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | TELEOST FISH ; GONADAL DIFFERENTIATION ; CAMPOMELIC DYSPLASIA ; TESTIS DEVELOPMENT ; GENE STRUCTURE ; SRY ; LATIPES ; MEDAKA ; MICE |
| 收录类别 | SCI |
| 资助信息 | State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [U0631007]; '863' High Technology Project [2006AA10Z141] |
| 语种 | 英语 |
| WOS记录号 | WOS:000280735200042 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13597]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Zhong Shan Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Yu-Shan,Hu, Wei,Liu, Xiao-Chun,et al. Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides)[J]. AQUACULTURE,2010,306(1-4):322-328. |
| APA | Luo, Yu-Shan,Hu, Wei,Liu, Xiao-Chun,Lin, Hao-Ran,&Zhu, Zuo-Yan.(2010).Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides).AQUACULTURE,306(1-4),322-328. |
| MLA | Luo, Yu-Shan,et al."Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides)".AQUACULTURE 306.1-4(2010):322-328. |
入库方式： OAI收割
来源：水生生物研究所
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