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Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)
文献类型：期刊论文
| 作者 | Wu, Shangong1,2; Gao, Tianheng3; Zheng, Yingzhen1,2,4; Wang, Weiwei1,2,4; Cheng, Yingyin1,2,4; Wang, Guitang1,2,4 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2010-05-24 |
| 卷号 | 303期号:1-4页码:1-7 |
| 关键词 | Intestinal microflora Intestinal mucus 16S rDNA Sequencing Biodiversity Yellow catfish |
| ISSN号 | 0044-8486 |
| 通讯作者 | Wang, GT, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | In this study, traditional culture-based techniques and the 16S rDNA sequencing method were used to investigate the microbial community of the intestinal contents and mucosal layer in the intestine of yellow catfish (Pelteobagrus fulvidraco). Eleven phylotypes were detected from culturable microbiota, and their closest relatives were Plesiomonas, Yersinia, Enterobacter, Shewanella, Aeromonas, Vibrio, and Myroides. Forty-four phylotypes were retrieved from 100 positive clones from intestinal contents (library C), and 21 phylotypes were detected in the 57 positive clones from intestinal mucus (library M), most of which were affiliated with Proteobacteria (>50% of the total). However, the bacterial groups OP10 and Actinobacteria detected in library C were not found in library M, suggesting that the abundance and diversity of bacterial populations in mucus might be different from the microbiota in gut contents, and that some microbial species poorly colonized the gut mucosal layer. (C) 2010 Published by Elsevier B.V. |
| 英文摘要 | In this study, traditional culture-based techniques and the 16S rDNA sequencing method were used to investigate the microbial community of the intestinal contents and mucosal layer in the intestine of yellow catfish (Pelteobagrus fulvidraco). Eleven phylotypes were detected from culturable microbiota, and their closest relatives were Plesiomonas, Yersinia, Enterobacter, Shewanella, Aeromonas, Vibrio, and Myroides. Forty-four phylotypes were retrieved from 100 positive clones from intestinal contents (library C), and 21 phylotypes were detected in the 57 positive clones from intestinal mucus (library M), most of which were affiliated with Proteobacteria (>50% of the total). However, the bacterial groups OP10 and Actinobacteria detected in library C were not found in library M, suggesting that the abundance and diversity of bacterial populations in mucus might be different from the microbiota in gut contents, and that some microbial species poorly colonized the gut mucosal layer. (C) 2010 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; 16S RIBOSOMAL-RNA ; RAINBOW-TROUT ; EDWARDSIELLA-ICTALURI ; PHYLOGENETIC ANALYSIS ; BACTERIAL-FLORA ; MOLECULAR CHARACTERIZATION ; SP-NOV ; MICROFLORA ; FISH |
| 收录类别 | SCI |
| 资助信息 | earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49-15]; National Program on Key Basic Research [2009CB118705] |
| 语种 | 英语 |
| WOS记录号 | WOS:000278152100001 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13613]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Shangong,Gao, Tianheng,Zheng, Yingzhen,et al. Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)[J]. AQUACULTURE,2010,303(1-4):1-7. |
| APA | Wu, Shangong,Gao, Tianheng,Zheng, Yingzhen,Wang, Weiwei,Cheng, Yingyin,&Wang, Guitang.(2010).Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco).AQUACULTURE,303(1-4),1-7. |
| MLA | Wu, Shangong,et al."Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco)".AQUACULTURE 303.1-4(2010):1-7. |
入库方式： OAI收割
来源：水生生物研究所
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