Viral Envelope Protein 53R Gene Highly Specific Silencing and Iridovirus Resistance in Fish Cells by AmiRNA
文献类型：期刊论文
| 作者 | Kim, Yu-Sin1,2; Ke, Fei1; Lei, Xiao-Ying1; Zhu, Rong1; Zhang, Qi-Ya1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2010-04-23 |
| 卷号 | 5期号:4页码:- |
| 关键词 | RANA-GRYLIO-VIRUS RNA INTERFERENCE PARALICHTHYS-OLIVACEUS FUNCTIONAL-CHARACTERIZATION EXPRESSION LINE INHIBITION SYSTEM SIRNA REPLICATION |
| ISSN号 | 1932-6203 |
| 通讯作者 | Kim, YS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 中文摘要 | Background: Envelope protein 53R was identified from frog Rana grylio virus (RGV), a member of the family Iridoviridae, and it plays an important role in the virus assembly. Although inhibition of iridovirus major capsid protein (MCP) by small hairpin RNAs (shRNAs) has been shown to cause resistance to viral infection in vitro, RNA interference (RNAi) to inhibit aquatic animal virus envelope protein gene product has not been reported. Methodology: We devised artificial microRNAs (amiRNAs) that target a viral envelope protein gene RGV 53R. By incorporating sequences encoding amiRNAs specific to 53R of RGV into pre-miRNA155 (pSM155) vectors, which use the backbone of natural miR-155 sequence and could intracellularly express 53R-targeted pre-amiRNAs. The pre-amiRNAs could be processed by the RNase III-like enzyme Dicer into 21-25 nt amiRNAs (amiR-53Rs) in fish cell lines. The levels of 53R expression were analyzed through real-time PCR and RGV virions assembly were observed by electronic microscopy in fish cells transfected with or without amiR-53Rs at 72 h of RGV infection. Conclusion/Significance: The results argue that viral envelope protein RGV 53R can be silenced and the virions assembly was deficient by amiR-53R-1, and further identified the first amiRNA of envelope protein gene from iridovirus that was able to cause resistance to virus infection in fish cells. The data demonstrate that the viral infection is efficiently suppressed (58%) by amiR-53R-1 targeting positon 36-57 of RGV 53R. Moreover, electron microscopic observations revealed virion assembly defect or reduced virions assembly capacity was closely correlated to expression of amiR-53R-1. Based on real time PCR of the Mx gene, we found no evidence of activation of IFN by amiR-53R-1. |
| 英文摘要 | Background: Envelope protein 53R was identified from frog Rana grylio virus (RGV), a member of the family Iridoviridae, and it plays an important role in the virus assembly. Although inhibition of iridovirus major capsid protein (MCP) by small hairpin RNAs (shRNAs) has been shown to cause resistance to viral infection in vitro, RNA interference (RNAi) to inhibit aquatic animal virus envelope protein gene product has not been reported. |
| WOS标题词 | Science & Technology |
| 学科主题 | Biology; Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | RANA-GRYLIO-VIRUS ; RNA INTERFERENCE ; PARALICHTHYS-OLIVACEUS ; FUNCTIONAL-CHARACTERIZATION ; EXPRESSION ; LINE ; INHIBITION ; SYSTEM ; SIRNA ; REPLICATION |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 30800854]; Agriculture Commonweal Scientific Research Plan [200803013]; China Postdoctoral Science Foundation [20080440975] |
| 语种 | 英语 |
| WOS记录号 | WOS:000277079300007 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13627]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Kim II Sung Univ, Coll Life Sci, Pyongyang, North Korea |
| 推荐引用方式 GB/T 7714 | Kim, Yu-Sin,Ke, Fei,Lei, Xiao-Ying,et al. Viral Envelope Protein 53R Gene Highly Specific Silencing and Iridovirus Resistance in Fish Cells by AmiRNA[J]. PLOS ONE,2010,5(4):-. |
| APA | Kim, Yu-Sin,Ke, Fei,Lei, Xiao-Ying,Zhu, Rong,&Zhang, Qi-Ya.(2010).Viral Envelope Protein 53R Gene Highly Specific Silencing and Iridovirus Resistance in Fish Cells by AmiRNA.PLOS ONE,5(4),-. |
| MLA | Kim, Yu-Sin,et al."Viral Envelope Protein 53R Gene Highly Specific Silencing and Iridovirus Resistance in Fish Cells by AmiRNA".PLOS ONE 5.4(2010):-. |
入库方式： OAI收割
来源：水生生物研究所
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