Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs
文献类型：期刊论文
| 作者 | Qi, Zhitao1,2; Nie, Pin1; Secombes, Chris J.3; Zou, Jun3 |
| 刊名 | JOURNAL OF IMMUNOLOGY
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 184期号:9页码:5038-5046 |
| 关键词 | INTERFERON GENES RECEPTOR IDENTIFICATION ZEBRAFISH VERTEBRATES FAMILY FISH EXPRESSION CYTOKINES SYSTEM |
| ISSN号 | 0022-1767 |
| 通讯作者 | Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Type I and III IFNs are structurally related cytokines with similar antiviral functions. They have different genomic organizations and bind to distinct receptor complexes. It has been vigorously debated whether the recently identified intron containing IFN genes in fish and amphibians belong to the type I or HI IFN family or diverged from a common ancestral gene, that subsequently gave rise to both types. In this report, we have identified intron containing type III IFN genes that are tandemly linked in the Xenopus tropicalis genome and hence demonstrate for the first time that intron containing type I and III genes diverged relatively early in vertebrate evolution, and at least by the appearance of early tetrapods, a transition period when vertebrates migrated from an aquatic environment to land. Our data also suggest that the intronless type I IFN genes seen in reptiles, birds, and mammals have originated from a type I IFN transcript via a retroposition event that led to the disappearance of intron-containing type I IFN genes in modern vertebrates. In vivo and in vitro studies in this paper show that the Xenopus type III IFNs and their cognate receptor are ubiquitously expressed in tissues and primary splenocytes and can be upregulated by stimulation with synthetic double-stranded RNA, suggesting they are involved in antiviral defense in amphibians. The Journal of Immunology, 2010, 184: 5038-5046. |
| 英文摘要 | Type I and III IFNs are structurally related cytokines with similar antiviral functions. They have different genomic organizations and bind to distinct receptor complexes. It has been vigorously debated whether the recently identified intron containing IFN genes in fish and amphibians belong to the type I or HI IFN family or diverged from a common ancestral gene, that subsequently gave rise to both types. In this report, we have identified intron containing type III IFN genes that are tandemly linked in the Xenopus tropicalis genome and hence demonstrate for the first time that intron containing type I and III genes diverged relatively early in vertebrate evolution, and at least by the appearance of early tetrapods, a transition period when vertebrates migrated from an aquatic environment to land. Our data also suggest that the intronless type I IFN genes seen in reptiles, birds, and mammals have originated from a type I IFN transcript via a retroposition event that led to the disappearance of intron-containing type I IFN genes in modern vertebrates. In vivo and in vitro studies in this paper show that the Xenopus type III IFNs and their cognate receptor are ubiquitously expressed in tissues and primary splenocytes and can be upregulated by stimulation with synthetic double-stranded RNA, suggesting they are involved in antiviral defense in amphibians. The Journal of Immunology, 2010, 184: 5038-5046. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology |
| 类目[WOS] | Immunology |
| 研究领域[WOS] | Immunology |
| 关键词[WOS] | INTERFERON GENES ; RECEPTOR ; IDENTIFICATION ; ZEBRAFISH ; VERTEBRATES ; FAMILY ; FISH ; EXPRESSION ; CYTOKINES ; SYSTEM |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]; Royal Society of Edinburgh |
| 语种 | 英语 |
| WOS记录号 | WOS:000277093000056 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13629]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Yancheng Inst Technol, Chem & Biol Engn Coll, Jiangsu, Peoples R China 3.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Inst Biol & Environm Sci, Aberdeen, Scotland |
| 推荐引用方式 GB/T 7714 | Qi, Zhitao,Nie, Pin,Secombes, Chris J.,et al. Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs[J]. JOURNAL OF IMMUNOLOGY,2010,184(9):5038-5046. |
| APA | Qi, Zhitao,Nie, Pin,Secombes, Chris J.,&Zou, Jun.(2010).Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs.JOURNAL OF IMMUNOLOGY,184(9),5038-5046. |
| MLA | Qi, Zhitao,et al."Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs".JOURNAL OF IMMUNOLOGY 184.9(2010):5038-5046. |
入库方式： OAI收割
来源：水生生物研究所
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