Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella
文献类型：期刊论文
| 作者 | Chen, W. Q.1,2,3; Xu, Q. Q.2,3; Chang, M. X.2,3; Zou, J.4; Secombes, C. J.4; Peng, K. M.1; Nie, P.2,3 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2010-04-15 |
| 卷号 | 134期号:3-4页码:199-207 |
| 关键词 | Grass carp IFN-gamma related gene Reovirus Peptidoglycan Nucleotide oligomerization domain |
| ISSN号 | 0165-2427 |
| 通讯作者 | Peng, KM, Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China |
| 中文摘要 | Interferon gamma (IFN-gamma), the only member of the type 11 class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gamma rel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gamma rel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gamma rel (gcIFN-gamma rel) showed 63% and 50% identities to zebrafish and common carp IFN-gamma rel (previously termed as IFN-gamma 1), respectively. The IFN-gamma rel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2 kb of genomic sequence. The gcIFN-gamma rel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gamma rel in spleen. The expression of gcIFN-gamma rel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gamma rel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gamma rel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gamma rel was possibly activated in a NOD2-dependent mechanism. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | Interferon gamma (IFN-gamma), the only member of the type 11 class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gamma rel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gamma rel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gamma rel (gcIFN-gamma rel) showed 63% and 50% identities to zebrafish and common carp IFN-gamma rel (previously termed as IFN-gamma 1), respectively. The IFN-gamma rel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2 kb of genomic sequence. The gcIFN-gamma rel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gamma rel in spleen. The expression of gcIFN-gamma rel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gamma rel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gamma rel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gamma rel was possibly activated in a NOD2-dependent mechanism. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; TOLL-LIKE RECEPTOR ; INTERFERON-GAMMA ; ATLANTIC SALMON ; CHANNEL CATFISH ; DANIO-RERIO ; IDENTIFICATION ; RESISTANCE ; FISH ; ZEBRAFISH |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh |
| 语种 | 英语 |
| WOS记录号 | WOS:000276518700007 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13633]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China 4.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland |
| 推荐引用方式 GB/T 7714 | Chen, W. Q.,Xu, Q. Q.,Chang, M. X.,et al. Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,134(3-4):199-207. |
| APA | Chen, W. Q..,Xu, Q. Q..,Chang, M. X..,Zou, J..,Secombes, C. J..,...&Nie, P..(2010).Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,134(3-4),199-207. |
| MLA | Chen, W. Q.,et al."Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 134.3-4(2010):199-207. |
入库方式： OAI收割
来源：水生生物研究所
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