The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella
文献类型：期刊论文
| 作者 | Xu, Qiao Q.1,2,3; Chang, Ming X.1; Xiao, Fan S.1; Huang, Bei1; Nie, Pin1 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2010-04-15 |
| 卷号 | 134期号:3-4页码:269-278 |
| 关键词 | IRF-5 Genome Expression GCHV Grass carp Ctenopharyngodon idella |
| ISSN号 | 0165-2427 |
| 通讯作者 | Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenophonyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075 bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenophonyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075 bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | INTERFERON REGULATORY FACTOR ; FISH SINIPERCA-CHUATSI ; MOLECULAR-CLONING ; RAINBOW-TROUT ; TRANSCRIPTION ; ACTIVATION ; ZEBRAFISH ; INDUCTION ; SEQUENCE ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276518700015 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13635]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China 3.Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qiao Q.,Chang, Ming X.,Xiao, Fan S.,et al. The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,134(3-4):269-278. |
| APA | Xu, Qiao Q.,Chang, Ming X.,Xiao, Fan S.,Huang, Bei,&Nie, Pin.(2010).The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,134(3-4),269-278. |
| MLA | Xu, Qiao Q.,et al."The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 134.3-4(2010):269-278. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。