Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization
文献类型：期刊论文
| 作者 | Li, N.1,2,3; Zhang, J.1,2,3; Zhang, L. Q.1,2,3; Nie, P.1,2 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 33期号:5页码:403-412 |
| 关键词 | Flavobacterium columnare pathogen suppression subtractive hybridization SSH virulence |
| ISSN号 | 0140-7775 |
| 通讯作者 | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | Flavobacterium columnare is the causative agent of columnaris disease. Different genetic groups of F. columnare show to some extent different degrees of virulence. To identify genetic differences between the high virulence strain G(4) and the low virulence strain G(18) of F. columnare, suppression subtractive hybridization was used. A total of 46 genes were identified from the virulent strain G(4), 35 of which showed some degree of homology with known proteins and can be classified into 11 categories: DNA replication or recombination proteins, inorganic ion transport proteins, outer membrane proteins, enterotoxin, binding proteins, YD repeat proteins, transposase, chaperon, signal transduction-related proteins, regulatory proteins, metabolism-related proteins. Several putative virulence factors identified in other bacteria could also be identified in the virulent strain G(4), such as ferrous iron transport protein, TonB-dependent receptor, transposases, as well as ABC transporter permease protein. The flanking region of a putative transposase ISFclI was analysed, and a putative Rhs element was located at the downstream of the putative transposase. The analysis of isfclI gene in 24 strains of F. columnare isolated in China revealed that 11 strains have isfclI, and all the strains from Zhaoqing, Anhui and Qingjiang have isfclI. |
| 英文摘要 | Flavobacterium columnare is the causative agent of columnaris disease. Different genetic groups of F. columnare show to some extent different degrees of virulence. To identify genetic differences between the high virulence strain G(4) and the low virulence strain G(18) of F. columnare, suppression subtractive hybridization was used. A total of 46 genes were identified from the virulent strain G(4), 35 of which showed some degree of homology with known proteins and can be classified into 11 categories: DNA replication or recombination proteins, inorganic ion transport proteins, outer membrane proteins, enterotoxin, binding proteins, YD repeat proteins, transposase, chaperon, signal transduction-related proteins, regulatory proteins, metabolism-related proteins. Several putative virulence factors identified in other bacteria could also be identified in the virulent strain G(4), such as ferrous iron transport protein, TonB-dependent receptor, transposases, as well as ABC transporter permease protein. The flanking region of a putative transposase ISFclI was analysed, and a putative Rhs element was located at the downstream of the putative transposase. The analysis of isfclI gene in 24 strains of F. columnare isolated in China revealed that 11 strains have isfclI, and all the strains from Zhaoqing, Anhui and Qingjiang have isfclI. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | OUTER-MEMBRANE ; FLEXIBACTER-COLUMNARIS ; ESCHERICHIA-COLI ; TROPICAL FISH ; PROTEIN ; PATHOGENICITY ; GENOMOVARS ; DIVERSITY ; ARCHAEA ; BRAZIL |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Programme of China [2009CB118703] |
| 语种 | 英语 |
| WOS记录号 | WOS:000276465300003 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13637]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, N.,Zhang, J.,Zhang, L. Q.,et al. Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization[J]. JOURNAL OF FISH DISEASES,2010,33(5):403-412. |
| APA | Li, N.,Zhang, J.,Zhang, L. Q.,&Nie, P..(2010).Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization.JOURNAL OF FISH DISEASES,33(5),403-412. |
| MLA | Li, N.,et al."Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization".JOURNAL OF FISH DISEASES 33.5(2010):403-412. |
入库方式： OAI收割
来源：水生生物研究所
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