Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos
文献类型：期刊论文
| 作者 | Hong, Ni1; Li, Mingyou1; Zeng, Zhiqiang1; Yi, Meisheng1; Deng, Jiaorong1; Gui, Jianfang2; Winkler, Christoph1; Schartl, Manfred3; Hong, Yunhan1,2 |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 67期号:7页码:1189-1202 |
| 关键词 | Chimera ES cell Host accessibility gamma-Irradiation Medaka |
| ISSN号 | 1420-682X |
| 通讯作者 | Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore |
| 中文摘要 | Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i (1) , i (3) and af), only strain i (1) generated pigmented chimeras. Strikingly, this accessibility is completely lost in i (1) but acquired in i (3) after host gamma-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production. |
| 英文摘要 | Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i (1) , i (3) and af), only strain i (1) generated pigmented chimeras. Strikingly, this accessibility is completely lost in i (1) but acquired in i (3) after host gamma-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | PRIMORDIAL GERM-CELLS ; VASA-LIKE GENE ; ORYZIAS-LATIPES ; IN-VITRO ; ES CELLS ; FISH ; ESTABLISHMENT ; ZEBRAFISH ; CHIMERAS ; DIFFERENTIATION |
| 收录类别 | SCI |
| 资助信息 | Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union |
| 语种 | 英语 |
| WOS记录号 | WOS:000275419800013 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13643]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore 2.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Univ Wurzburg, Bioctr, D-97074 Wurzburg, Germany |
| 推荐引用方式 GB/T 7714 | Hong, Ni,Li, Mingyou,Zeng, Zhiqiang,et al. Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2010,67(7):1189-1202. |
| APA | Hong, Ni.,Li, Mingyou.,Zeng, Zhiqiang.,Yi, Meisheng.,Deng, Jiaorong.,...&Hong, Yunhan.(2010).Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos.CELLULAR AND MOLECULAR LIFE SCIENCES,67(7),1189-1202. |
| MLA | Hong, Ni,et al."Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos".CELLULAR AND MOLECULAR LIFE SCIENCES 67.7(2010):1189-1202. |
入库方式： OAI收割
来源：水生生物研究所
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