Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection
文献类型：期刊论文
| 作者 | Xiao, Zhan-Gang1,3; Liu, Hong2; Fu, Jian-Ping1,3; Hu, Wei1; Wang, Ya-Ping1; Guo, Qiong-Lin1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 28期号:2页码:362-371 |
| 关键词 | SOCS-3 Common carp Embryogenesis GH-transgenic carp Spring viraemia of carp virus (SVCV) |
| ISSN号 | 1050-4648 |
| 通讯作者 | Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | As a member of a newly discovered protein family, the suppressor of cytokine signalling 3 (SOCS-3) has been shown to regulate the responses of many immune cytokines in a negative auto-regulatory manner. The full-length cDNA of common carp SOCS-3 was 1603 bp and contained a 630 bp open reading frame (ORF) coding for a protein of 209 amino acids. Carp SOCS-3 molecule was well conserved especially in the SRC homology 2 (SH2) and the SOCS box. The kinase inhibitory region (KIR) and ESS domains, upstream of the SH2 domain, were conserved in carp SOCS-3, except for a specific insertion (PHRYK) in the KIR domain at the N-terminal region. Three conserved cysteine (Cys-102, 124 and 193) residues, and one additional cysteine (Cys-168) residue, were also found in carp SOCS-3. The 2015 bp genomic DNA of carp SOCS-3 contained two exons and one intron. Phylogenetic analysis showed that carp SOCS-3 sequence grouped with other known fish SOCS-3 sequences with zebrafish SOCS-3 as the closest neighbour. RT-PCR analysis showed that carp SOCS-3 was initially expressed at 4 h pf (post-fertilization) and gradually increased up to 4 w pf during embryogenesis. By RT-qPCR analysis, carp SOCS-3 gene was predominantly detected in gill, head kidney, thymus and skin, followed by spleen and peripheral blood, lower expression level was detected in kidney, intestine, liver and muscle; the SOCS-3 transcript was significantly increased in thymus, head kidney, spleen and intestine of GH (growth hormone)-transgenic carp; after SVCV (spring viraemia of carp virus) infection, the carp SOCS-3 transcript was significantly up-regulated in gill, intestine, thymus, spleen, head kidney and kidney tissues in a time-dependent manner. These results suggest that teleost SOCS-3 may play an active role in the modulation of viral-induced innate immune response and in preventing the overaction of some cytokines with viral stimulation. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved. |
| 英文摘要 | As a member of a newly discovered protein family, the suppressor of cytokine signalling 3 (SOCS-3) has been shown to regulate the responses of many immune cytokines in a negative auto-regulatory manner. The full-length cDNA of common carp SOCS-3 was 1603 bp and contained a 630 bp open reading frame (ORF) coding for a protein of 209 amino acids. Carp SOCS-3 molecule was well conserved especially in the SRC homology 2 (SH2) and the SOCS box. The kinase inhibitory region (KIR) and ESS domains, upstream of the SH2 domain, were conserved in carp SOCS-3, except for a specific insertion (PHRYK) in the KIR domain at the N-terminal region. Three conserved cysteine (Cys-102, 124 and 193) residues, and one additional cysteine (Cys-168) residue, were also found in carp SOCS-3. The 2015 bp genomic DNA of carp SOCS-3 contained two exons and one intron. Phylogenetic analysis showed that carp SOCS-3 sequence grouped with other known fish SOCS-3 sequences with zebrafish SOCS-3 as the closest neighbour. RT-PCR analysis showed that carp SOCS-3 was initially expressed at 4 h pf (post-fertilization) and gradually increased up to 4 w pf during embryogenesis. By RT-qPCR analysis, carp SOCS-3 gene was predominantly detected in gill, head kidney, thymus and skin, followed by spleen and peripheral blood, lower expression level was detected in kidney, intestine, liver and muscle; the SOCS-3 transcript was significantly increased in thymus, head kidney, spleen and intestine of GH (growth hormone)-transgenic carp; after SVCV (spring viraemia of carp virus) infection, the carp SOCS-3 transcript was significantly up-regulated in gill, intestine, thymus, spleen, head kidney and kidney tissues in a time-dependent manner. These results suggest that teleost SOCS-3 may play an active role in the modulation of viral-induced innate immune response and in preventing the overaction of some cytokines with viral stimulation. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | INFLUENZA-A VIRUS ; CYTOKINE SIGNALING-3 ; GROWTH-HORMONE ; CYPRINUS-CARPIO ; ANALYSIS SOFTWARE ; SPRING VIREMIA ; SUPPRESSOR ; RECEPTOR ; IDENTIFICATION ; PROTEINS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] |
| 语种 | 英语 |
| WOS记录号 | WOS:000274721300015 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13647]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Zhan-Gang,Liu, Hong,Fu, Jian-Ping,et al. Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):362-371. |
| APA | Xiao, Zhan-Gang,Liu, Hong,Fu, Jian-Ping,Hu, Wei,Wang, Ya-Ping,&Guo, Qiong-Lin.(2010).Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection.FISH & SHELLFISH IMMUNOLOGY,28(2),362-371. |
| MLA | Xiao, Zhan-Gang,et al."Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):362-371. |
入库方式： OAI收割
来源：水生生物研究所
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