Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei
文献类型：期刊论文
| 作者 | Li, Ai-Hua3; Na, Byoung-Kuk4,5; Ahn, Sung-Kyu1,2; Cho, Shin-Hyeong6; Pak, Jhang-Ho7; Park, Yun-Kyu1,2; Kim, Tong-Soo1,2 |
| 刊名 | PARASITOLOGY RESEARCH
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 106期号:3页码:627-635 |
| 关键词 | PARASITE ONCHOCERCA-VOLVULUS |
| ISSN号 | 0932-0113 |
| 通讯作者 | Kim, TS, Inha Univ, Sch Med, Dept Parasitol, Inchon 400712, South Korea |
| 中文摘要 | Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a gene encoding the copper/zinc-superoxide dismutase of S. erinacei (SeCuZnSOD) and partially characterized the biochemical and functional properties of the enzyme. The open reading frame of SeCuZnSOD was 465 bp that encodes 154 amino acids. The characteristic amino acid residues and motifs required for coordinating copper and zinc enzymatic function were well conserved. The genomic length of the SeCuZnSOD was 1,985 bp consisting of three exons that are separated by two introns. SeCuZnSOD is a typical cytosolic form which shares similar biochemical properties, including broad pH optima and inhibition profile by KCN and H2O2, with cytosolic Cu/Zn-SODs of other organisms. SeCuZnSOD was functionally expressed in both S. erinacei plerocercoid larvae and adult worms, and its expression level was significantly increased when the plerocercoid larvae were treated with paraquat. The enzyme may play essential roles for survival of the parasite not only by protecting itself from endogenous oxidative stress, but also by detoxifying oxidative killing of the parasite by host immune effector cells. |
| 英文摘要 | Spirometra erinacei is a pseudophyllidean tapeworm which inhabits the intestines of cats and dogs. The infections are usually asymptomatic in these animals, but the infection of the plerocercoid larvae of the parasite, spargana, cause sparganosis in other vertebrates, including human. In this study, we identified a gene encoding the copper/zinc-superoxide dismutase of S. erinacei (SeCuZnSOD) and partially characterized the biochemical and functional properties of the enzyme. The open reading frame of SeCuZnSOD was 465 bp that encodes 154 amino acids. The characteristic amino acid residues and motifs required for coordinating copper and zinc enzymatic function were well conserved. The genomic length of the SeCuZnSOD was 1,985 bp consisting of three exons that are separated by two introns. SeCuZnSOD is a typical cytosolic form which shares similar biochemical properties, including broad pH optima and inhibition profile by KCN and H(2)O(2), with cytosolic Cu/Zn-SODs of other organisms. SeCuZnSOD was functionally expressed in both S. erinacei plerocercoid larvae and adult worms, and its expression level was significantly increased when the plerocercoid larvae were treated with paraquat. The enzyme may play essential roles for survival of the parasite not only by protecting itself from endogenous oxidative stress, but also by detoxifying oxidative killing of the parasite by host immune effector cells. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Parasitology |
| 类目[WOS] | Parasitology |
| 研究领域[WOS] | Parasitology |
| 关键词[WOS] | PARASITE ONCHOCERCA-VOLVULUS ; SCHISTOSOMA-MANSONI ; MOLECULAR-CLONING ; FASCIOLA-HEPATICA ; DIFFERENTIAL EXPRESSION ; BRUGIA-MALAYI ; MESSENGER-RNA ; TAENIA-SOLIUM ; PROTECTION ; ENZYMES |
| 收录类别 | SCI |
| 资助信息 | Inha University Research Grant [INHA-37466-01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000274252400011 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13649]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Inha Univ, Sch Med, Dept Parasitol, Inchon 400712, South Korea 2.Inha Univ, Sch Med, Inha Res Inst Med Sci, Inchon 400712, South Korea 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.Gyeongsang Natl Univ, Sch Med, Dept Parasitol, Biomed Ctr BK21, Jinju 660751, South Korea 5.Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Jinju 660751, South Korea 6.Ctr Dis Control & Prevent, Natl Inst Hlth, Dept Malaria & Parasit Dis, Seoul 122701, South Korea 7.Univ Ulsan, Coll Med, Asan Med Ctr, Asan Inst Life Sci, Seoul 138736, South Korea |
| 推荐引用方式 GB/T 7714 | Li, Ai-Hua,Na, Byoung-Kuk,Ahn, Sung-Kyu,et al. Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei[J]. PARASITOLOGY RESEARCH,2010,106(3):627-635. |
| APA | Li, Ai-Hua.,Na, Byoung-Kuk.,Ahn, Sung-Kyu.,Cho, Shin-Hyeong.,Pak, Jhang-Ho.,...&Kim, Tong-Soo.(2010).Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei.PARASITOLOGY RESEARCH,106(3),627-635. |
| MLA | Li, Ai-Hua,et al."Functional expression and characterization of a cytosolic copper/zinc-superoxide dismutase of Spirometra erinacei".PARASITOLOGY RESEARCH 106.3(2010):627-635. |
入库方式： OAI收割
来源：水生生物研究所
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