Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Shi, Yan1; Zhu, Xin-Ping1,3; Yin, Jing-Kui1; Zhang, Qi-Ya2; Gui, Jian-Fang2 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 37期号:3页码:1483-1493 |
| 关键词 | Interferon regulator factor-1 (IRF-1) Epinephelus coioides Genomic structure Inductive expression Subcellular localization |
| ISSN号 | 0301-4851 |
| 通讯作者 | Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China |
| 中文摘要 | Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper. |
| 英文摘要 | Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CARASSIUS-AURATUS L. ; UV-INACTIVATED VIRUS ; IFN-INDUCIBLE GENES ; MOLECULAR-CLONING ; PARALICHTHYS-OLIVACEUS ; EXPRESSION ANALYSIS ; SUBCELLULAR-LOCALIZATION ; TRANSCRIPTION FACTOR ; JAPANESE FLOUNDER ; FISH CELLS |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000274212500043 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13651]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yan,Zhu, Xin-Ping,Yin, Jing-Kui,et al. Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)[J]. MOLECULAR BIOLOGY REPORTS,2010,37(3):1483-1493. |
| APA | Shi, Yan,Zhu, Xin-Ping,Yin, Jing-Kui,Zhang, Qi-Ya,&Gui, Jian-Fang.(2010).Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides).MOLECULAR BIOLOGY REPORTS,37(3),1483-1493. |
| MLA | Shi, Yan,et al."Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)".MOLECULAR BIOLOGY REPORTS 37.3(2010):1483-1493. |
入库方式： OAI收割
来源：水生生物研究所
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