Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization
文献类型：期刊论文
| 作者 | Shi, Yan1; Zhao, Zhe2; Yin, Jing-Kui1; Zhu, Xin-Ping1,3; Chen, Kun-Ci1; Pan, De-Bo1; Gui, Jian-Fang4 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 155期号:2页码:110-117 |
| 关键词 | Interferon regulator factor-2 (IRF-2) Epinephelus coioides Genomic structure Inductive expression Subcellular localization |
| ISSN号 | 1096-4959 |
| 通讯作者 | Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China |
| 中文摘要 | Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper. (C) 2009 Elsevier Inc. All rights reserved. |
| 英文摘要 | Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper. (C) 2009 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | FACTOR-I ; TRANSCRIPTIONAL REPRESSION ; MOLECULAR CHARACTERIZATION ; FUNCTIONAL-ANALYSIS ; RAINBOW-TROUT ; MESSENGER-RNA ; DNA-SEQUENCES ; HOST-DEFENSE ; IRF-1 ; ACTIVATION |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000273927000003 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13655]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yan,Zhao, Zhe,Yin, Jing-Kui,et al. Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,155(2):110-117. |
| APA | Shi, Yan.,Zhao, Zhe.,Yin, Jing-Kui.,Zhu, Xin-Ping.,Chen, Kun-Ci.,...&Gui, Jian-Fang.(2010).Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,155(2),110-117. |
| MLA | Shi, Yan,et al."Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 155.2(2010):110-117. |
入库方式： OAI收割
来源：水生生物研究所
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