Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection
文献类型：期刊论文
| 作者 | Zhou, Xiuxia1,2; Feng, Hong1,2; Guo, Qionglin1; Dai, Heping1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 34期号:2页码:150-157 |
| 关键词 | CD9 Tetraspanin family cDNA cloning Bacterial infection Chinese soft-shelled turtle |
| ISSN号 | 0145-305X |
| 通讯作者 | Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146 bp and contained a 672 bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N-, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif. a potential N-linked glycosylation site and 10 cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to duck CD9. Most of the differences were found in the LEL Phylogenetic analysis showed that the turtle CD9 sequence clustered together with bird CD9 sequence. RT-PCR analysis showed that turtle CD9 was ubiquitously expressed in liver, spleen, kidney, heart, blood and intestine tissues of un-infected turtles. Real-time PCR analysis further indicated that after Aeromonas hydrophila infection, the turtle CD9 mRNA was up-regulated in various tissues at 8 h, and significantly up-regulated during 8 h to 7 d. These results indicated that turtle CD9 may be involved in anti-bacterial immune response. (C) 2009 Elsevier Ltd. All rights reserved. |
| 英文摘要 | In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146 bp and contained a 672 bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N-, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif. a potential N-linked glycosylation site and 10 cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to duck CD9. Most of the differences were found in the LEL Phylogenetic analysis showed that the turtle CD9 sequence clustered together with bird CD9 sequence. RT-PCR analysis showed that turtle CD9 was ubiquitously expressed in liver, spleen, kidney, heart, blood and intestine tissues of un-infected turtles. Real-time PCR analysis further indicated that after Aeromonas hydrophila infection, the turtle CD9 mRNA was up-regulated in various tissues at 8 h, and significantly up-regulated during 8 h to 7 d. These results indicated that turtle CD9 may be involved in anti-bacterial immune response. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | CELL-SURFACE ; MOLECULAR-CLONING ; TETRASPANIN CD9 ; EXTRACELLULAR DOMAIN ; PROTEINS ; ANTIGEN ; INTEGRINS ; SUPERFAMILY ; ACTIVATION ; ADHESION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] |
| 语种 | 英语 |
| WOS记录号 | WOS:000273147400009 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13661]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Xiuxia,Feng, Hong,Guo, Qionglin,et al. Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(2):150-157. |
| APA | Zhou, Xiuxia,Feng, Hong,Guo, Qionglin,&Dai, Heping.(2010).Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(2),150-157. |
| MLA | Zhou, Xiuxia,et al."Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.2(2010):150-157. |
入库方式： OAI收割
来源：水生生物研究所
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