cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii
文献类型：期刊论文
| 作者 | Xu, Qiaoqing1; Wang, Gailing2; Yuan, Hanwen1; Chai, Yi1; Xiao, Zhili1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 157期号:1页码:119-126 |
| 关键词 | Antibacterial peptide Theromacin Mussel Hemocyte Gram-positive bacteria |
| ISSN号 | 1096-4959 |
| 通讯作者 | Xu, QQ, Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China |
| 中文摘要 | Bivalve molluscs rely on the interaction between cellular and humoral factors for protection against potential pathogens. Antimicrobial peptides (AMPS) have been proven to be one of the most important humoral components that afford resistance to pathogen infection. The AMP gene to be identified was that encoding theromacin in the triangle-shell pearl mussel Hyriopsis cumingii (Hc theromacin); this gene was identified from a suppression subtractive hybridization library, and subsequently cloned by 3 ' and 5 ' rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). The full-length theromacin cDNA contains 547 bp, with a 294-bp open reading frame that encodes a 97-amino acid peptide, and the deduced peptide sequence contains a 61-amino acid putative mature peptide. The sequence also contains 10 cysteine residues. Reverse transcriptase (RT)-PCR analysis showed that Hc theromacin transcripts were constitutively expressed in the liver, foot, gill, adductor muscle, heart, mantle, intestine, and hemocytes, with the highest level in hemocytes. Theromacin mRNA levels were found to increase after challenge with Gram-positive and Gram-negative bacteria. After injection of the Gram-positive bacteria Staphylococcus aureus and Bifidobacterium bifidum, Hc theromacin expression showed the highest fold-change at 48 and 36 h after infection, respectively, and its levels decreased gradually thereafter. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved. |
| 英文摘要 | Bivalve molluscs rely on the interaction between cellular and humoral factors for protection against potential pathogens. Antimicrobial peptides (AMPS) have been proven to be one of the most important humoral components that afford resistance to pathogen infection. The AMP gene to be identified was that encoding theromacin in the triangle-shell pearl mussel Hyriopsis cumingii (Hc theromacin); this gene was identified from a suppression subtractive hybridization library, and subsequently cloned by 3 ' and 5 ' rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). The full-length theromacin cDNA contains 547 bp, with a 294-bp open reading frame that encodes a 97-amino acid peptide, and the deduced peptide sequence contains a 61-amino acid putative mature peptide. The sequence also contains 10 cysteine residues. Reverse transcriptase (RT)-PCR analysis showed that Hc theromacin transcripts were constitutively expressed in the liver, foot, gill, adductor muscle, heart, mantle, intestine, and hemocytes, with the highest level in hemocytes. Theromacin mRNA levels were found to increase after challenge with Gram-positive and Gram-negative bacteria. After injection of the Gram-positive bacteria Staphylococcus aureus and Bifidobacterium bifidum, Hc theromacin expression showed the highest fold-change at 48 and 36 h after infection, respectively, and its levels decreased gradually thereafter. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; MYTILUS-GALLOPROVINCIALIS ; MOLECULAR CHARACTERIZATION ; MARINE-INVERTEBRATES ; ARTHROPOD DEFENSIN ; INNATE IMMUNITY ; SHRIMP ; HEMOCYTES ; INVOLVEMENT ; PENAEIDINS |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] |
| 语种 | 英语 |
| WOS记录号 | WOS:000280027300016 |
| 公开日期 | 2010-12-23 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13663]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qiaoqing,Wang, Gailing,Yuan, Hanwen,et al. cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,157(1):119-126. |
| APA | Xu, Qiaoqing,Wang, Gailing,Yuan, Hanwen,Chai, Yi,&Xiao, Zhili.(2010).cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,157(1),119-126. |
| MLA | Xu, Qiaoqing,et al."cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 157.1(2010):119-126. |
入库方式： OAI收割
来源：水生生物研究所
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