Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains
文献类型：期刊论文
| 作者 | Chang, Mingxian1,2,3; Wang, Tiehui1; Nie, Pin2,3; Zou, Jun1; Secombes, Christopher J.1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 30期号:1页码:118-127 |
| 关键词 | Rainbow trout NOD2 Proinflammatory cytokine Apoptosis |
| ISSN号 | 1050-4648 |
| 通讯作者 | Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland ; c.secombes@abdn.ac.uk |
| 中文摘要 | Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | NF-KAPPA-B ; SALMON SALMO-SALAR ; ONCORHYNCHUS-MYKISS ; FAMILY-MEMBER ; IFN-GAMMA ; MURAMYL DIPEPTIDE ; INNATE IMMUNITY ; TELEOST FISH ; APOPTOSIS ; RECOGNITION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30830083]; Royal Society of Edinburgh ; EU |
| 语种 | 英语 |
| WOS记录号 | WOS:000286905800015 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15486]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Mingxian,Wang, Tiehui,Nie, Pin,et al. Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):118-127. |
| APA | Chang, Mingxian,Wang, Tiehui,Nie, Pin,Zou, Jun,&Secombes, Christopher J..(2011).Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains.FISH & SHELLFISH IMMUNOLOGY,30(1),118-127. |
| MLA | Chang, Mingxian,et al."Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):118-127. |
入库方式： OAI收割
来源：水生生物研究所
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